BD HorizonTM RB613 Streptavidin

Description

Multiparameter flow cytometric analyses using BD Horizon RB613 Streptavidin Left Plot: CD45R/B220 expression on viable Mouse splenic leukocytes. Mouse splenocytes were either labeled with Biotin Rat IgG2a kappa Isotype Control Cat. No. 553928; dashed line histogram or with Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553086; solid line histogram . The cells were then washed and secondarily stained with BD Horizon RB613 Streptavidin Cat. No. 571111 at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Right Plot: CD3 expression on Human peripheral blood lymphocytes. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were either labeled with Biotin Mouse IgG1, kappa Isotype Control Cat. No. 569597; dashed line histogram or with Biotin Mouse Anti-Human CD3 antibody solid line histogram; Cat. No. 555331 . The cells were then washed and secondarily stained with BD Horizon RB613 Streptavidin Cat. No. 571111 at 0.25 mug/test. The histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lympocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analyses using BD Horizon RB613 Streptavidin Left Plot: CD45R/B220 expression on viable Mouse splenic leukocytes. Mouse splenocytes were either labeled with Biotin Rat IgG2a kappa Isotype Control Cat. No. 553928; dashed line histogram or with Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553086; solid line histogram . The cells were then washed and secondarily stained with BD Horizon RB613 Streptavidin Cat. No. 571111 at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Right Plot: CD3 expression on Human peripheral blood lymphocytes. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were either labeled with Biotin Mouse IgG1, kappa Isotype Control Cat. No. 569597; dashed line histogram or with Biotin Mouse Anti-Human CD3 antibody solid line histogram; Cat. No. 555331 . The cells were then washed and secondarily stained with BD Horizon RB613 Streptavidin Cat. No. 571111 at 0.25 mug/test. The histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lympocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analyses using BD Horizon RB613 Streptavidin Left Plot: CD45R/B220 expression on viable Mouse splenic leukocytes. Mouse splenocytes were either labeled with Biotin Rat IgG2a kappa Isotype Control Cat. No. 553928; dashed line histogram or with Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553086; solid line histogram . The cells were then washed and secondarily stained with BD Horizon RB613 Streptavidin Cat. No. 571111 at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Right Plot: CD3 expression on Human peripheral blood lymphocytes. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were either labeled with Biotin Mouse IgG1, kappa Isotype Control Cat. No. 569597; dashed line histogram or with Biotin Mouse Anti-Human CD3 antibody solid line histogram; Cat. No. 555331 . The cells were then washed and secondarily stained with BD Horizon RB613 Streptavidin Cat. No. 571111 at 0.25 mug/test. The histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lympocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analyses using BD Horizon RB613 Streptavidin Left Plot: CD45R/B220 expression on viable Mouse splenic leukocytes. Mouse splenocytes were either labeled with Biotin Rat IgG2a kappa Isotype Control Cat. No. 553928; dashed line histogram or with Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553086; solid line histogram . The cells were then washed and secondarily stained with BD Horizon RB613 Streptavidin Cat. No. 571111 at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Right Plot: CD3 expression on Human peripheral blood lymphocytes. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were either labeled with Biotin Mouse IgG1, kappa Isotype Control Cat. No. 569597; dashed line histogram or with Biotin Mouse Anti-Human CD3 antibody solid line histogram; Cat. No. 555331 . The cells were then washed and secondarily stained with BD Horizon RB613 Streptavidin Cat. No. 571111 at 0.25 mug/test. The histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lympocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analyses using BD Horizon RB613 Streptavidin Left Plot: CD45R/B220 expression on viable Mouse splenic leukocytes. Mouse splenocytes were either labeled with Biotin Rat IgG2a kappa Isotype Control Cat. No. 553928; dashed line histogram or with Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553086; solid line histogram . The cells were then washed and secondarily stained with BD Horizon RB613 Streptavidin Cat. No. 571111 at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Right Plot: CD3 expression on Human peripheral blood lymphocytes. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were either labeled with Biotin Mouse IgG1, kappa Isotype Control Cat. No. 569597; dashed line histogram or with Biotin Mouse Anti-Human CD3 antibody solid line histogram; Cat. No. 555331 . The cells were then washed and secondarily stained with BD Horizon RB613 Streptavidin Cat. No. 571111 at 0.25 mug/test. The histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lympocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analyses using BD Horizon RB613 Streptavidin Left Plot: CD45R/B220 expression on viable Mouse splenic leukocytes. Mouse splenocytes were either labeled with Biotin Rat IgG2a kappa Isotype Control Cat. No. 553928; dashed line histogram or with Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553086; solid line histogram . The cells were then washed and secondarily stained with BD Horizon RB613 Streptavidin Cat. No. 571111 at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Right Plot: CD3 expression on Human peripheral blood lymphocytes. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were either labeled with Biotin Mouse IgG1, kappa Isotype Control Cat. No. 569597; dashed line histogram or with Biotin Mouse Anti-Human CD3 antibody solid line histogram; Cat. No. 555331 . The cells were then washed and secondarily stained with BD Horizon RB613 Streptavidin Cat. No. 571111 at 0.25 mug/test. The histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lympocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.