BD HorizonTM RB670 Mouse Anti-Human CD137 (4-1BB)

Description

Flow cytometric analysis of CD137 4-1BB expression on viable stimulated Human peripheral blood lymphocytes. Concanavalin A Con-A -stimulated 3 days peripheral blood mononuclear cells PBMC were stained with either BD Horizon RB670 Mouse IgG1, kappa Isotype Control Cat. No. 571784; dashed line histogram or BD Horizon RB670 Mouse Anti-Human CD137 4-1BB antibody Cat. No. 572251/572279; solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing the expression of CD137 4-1BB or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative activated lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Flow cytometric analysis of CD137 4-1BB expression on viable stimulated Human peripheral blood lymphocytes. Concanavalin A Con-A -stimulated 3 days peripheral blood mononuclear cells PBMC were stained with either BD Horizon RB670 Mouse IgG1, kappa Isotype Control Cat. No. 571784; dashed line histogram or BD Horizon RB670 Mouse Anti-Human CD137 4-1BB antibody Cat. No. 572251/572279; solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing the expression of CD137 4-1BB or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative activated lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Show More Flow cytometric analysis of CD137 4-1BB expression on viable stimulated Human peripheral blood lymphocytes. Concanavalin A Con-A -stimulated 3 days peripheral blood mononuclear cells PBMC were stained with either BD Horizon RB670 Mouse IgG1, kappa Isotype Control Cat. No. 571784; dashed line histogram or BD Horizon RB670 Mouse Anti-Human CD137 4-1BB antibody Cat. No. 572251/572279; solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing the expression of CD137 4-1BB or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative activated lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software.