BD HorizonTM RB670 Mouse Anti-Human CD45RA

Description

Multicolor flow cytometric analysis of CD45RA expression on Human peripheral blood leukocytes. Human whole blood was stained with BD Horizon RY703 Mouse Anti-Human CD45RO Cat. No. 571463; Bottom Plots and with either BD Horizon RB670 Mouse IgG1, kappa Isotype Control Cat. No. 571784; Left Plots or BD Horizon RB670 Mouse Anti-Human CD45RA antibody Cat. No. 571965/571966; Right Plots . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plots showing the correlated expression of CD45RA or Ig Isotype control staining versus either side light-scatter SSC-A signals Top Plots or CD45RO Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of either viable leukocytes or lymphocytes, respectively. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Multicolor flow cytometric analysis of CD45RA expression on Human peripheral blood leukocytes. Human whole blood was stained with BD Horizon RY703 Mouse Anti-Human CD45RO Cat. No. 571463; Bottom Plots and with either BD Horizon RB670 Mouse IgG1, kappa Isotype Control Cat. No. 571784; Left Plots or BD Horizon RB670 Mouse Anti-Human CD45RA antibody Cat. No. 571965/571966; Right Plots . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plots showing the correlated expression of CD45RA or Ig Isotype control staining versus either side light-scatter SSC-A signals Top Plots or CD45RO Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of either viable leukocytes or lymphocytes, respectively. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Show More Multicolor flow cytometric analysis of CD45RA expression on Human peripheral blood leukocytes. Human whole blood was stained with BD Horizon RY703 Mouse Anti-Human CD45RO Cat. No. 571463; Bottom Plots and with either BD Horizon RB670 Mouse IgG1, kappa Isotype Control Cat. No. 571784; Left Plots or BD Horizon RB670 Mouse Anti-Human CD45RA antibody Cat. No. 571965/571966; Right Plots . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plots showing the correlated expression of CD45RA or Ig Isotype control staining versus either side light-scatter SSC-A signals Top Plots or CD45RO Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of either viable leukocytes or lymphocytes, respectively. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software.