BD HorizonTM RB670 Mouse Anti-Mouse RORgammat

Description

Multicolor flow cytometric analysis of RORgammat expression in Mouse thymocytes. C57BL/6 Mouse thymocytes were stained with BD OptiBuild BV421 Rat Anti-Mouse CD4 Cat. No. 740007 and BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No. 563786 antibodies, and then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 . The fixed and permeabilized cells were then stained with either BD Horizon RB670 Mouse IgG2a, kappa Isotype Control Cat. No. 571958 or BD Horizon RB670 Mouse Anti-Mouse RORgammat antibody Cat. No. 571951/571952 at 0.125 mug/test. The overlapping histograms show the levels of either IgG2a Isotype Control Left Panel or RORgammat Right Panel staining in CD4+CD8- single-positive dashed line histograms or CD4+CD8+ double-positive solid line histograms thymocytes. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer and were spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of RORgammat expression in Mouse thymocytes. C57BL/6 Mouse thymocytes were stained with BD OptiBuild BV421 Rat Anti-Mouse CD4 Cat. No. 740007 and BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No. 563786 antibodies, and then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 . The fixed and permeabilized cells were then stained with either BD Horizon RB670 Mouse IgG2a, kappa Isotype Control Cat. No. 571958 or BD Horizon RB670 Mouse Anti-Mouse RORgammat antibody Cat. No. 571951/571952 at 0.125 mug/test. The overlapping histograms show the levels of either IgG2a Isotype Control Left Panel or RORgammat Right Panel staining in CD4+CD8- single-positive dashed line histograms or CD4+CD8+ double-positive solid line histograms thymocytes. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer and were spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of RORgammat expression in Mouse thymocytes. C57BL/6 Mouse thymocytes were stained with BD OptiBuild BV421 Rat Anti-Mouse CD4 Cat. No. 740007 and BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No. 563786 antibodies, and then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 . The fixed and permeabilized cells were then stained with either BD Horizon RB670 Mouse IgG2a, kappa Isotype Control Cat. No. 571958 or BD Horizon RB670 Mouse Anti-Mouse RORgammat antibody Cat. No. 571951/571952 at 0.125 mug/test. The overlapping histograms show the levels of either IgG2a Isotype Control Left Panel or RORgammat Right Panel staining in CD4+CD8- single-positive dashed line histograms or CD4+CD8+ double-positive solid line histograms thymocytes. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer and were spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of RORgammat expression in Mouse thymocytes. C57BL/6 Mouse thymocytes were stained with BD OptiBuild BV421 Rat Anti-Mouse CD4 Cat. No. 740007 and BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No. 563786 antibodies, and then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 . The fixed and permeabilized cells were then stained with either BD Horizon RB670 Mouse IgG2a, kappa Isotype Control Cat. No. 571958 or BD Horizon RB670 Mouse Anti-Mouse RORgammat antibody Cat. No. 571951/571952 at 0.125 mug/test. The overlapping histograms show the levels of either IgG2a Isotype Control Left Panel or RORgammat Right Panel staining in CD4+CD8- single-positive dashed line histograms or CD4+CD8+ double-positive solid line histograms thymocytes. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer and were spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of RORgammat expression in Mouse thymocytes. C57BL/6 Mouse thymocytes were stained with BD OptiBuild BV421 Rat Anti-Mouse CD4 Cat. No. 740007 and BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No. 563786 antibodies, and then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 . The fixed and permeabilized cells were then stained with either BD Horizon RB670 Mouse IgG2a, kappa Isotype Control Cat. No. 571958 or BD Horizon RB670 Mouse Anti-Mouse RORgammat antibody Cat. No. 571951/571952 at 0.125 mug/test. The overlapping histograms show the levels of either IgG2a Isotype Control Left Panel or RORgammat Right Panel staining in CD4+CD8- single-positive dashed line histograms or CD4+CD8+ double-positive solid line histograms thymocytes. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer and were spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of RORgammat expression in Mouse thymocytes. C57BL/6 Mouse thymocytes were stained with BD OptiBuild BV421 Rat Anti-Mouse CD4 Cat. No. 740007 and BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No. 563786 antibodies, and then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 . The fixed and permeabilized cells were then stained with either BD Horizon RB670 Mouse IgG2a, kappa Isotype Control Cat. No. 571958 or BD Horizon RB670 Mouse Anti-Mouse RORgammat antibody Cat. No. 571951/571952 at 0.125 mug/test. The overlapping histograms show the levels of either IgG2a Isotype Control Left Panel or RORgammat Right Panel staining in CD4+CD8- single-positive dashed line histograms or CD4+CD8+ double-positive solid line histograms thymocytes. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer and were spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific.