BD HorizonTM RB705 Hamster Anti-Mouse CD103 (Integrin alphaE)

Description

Multicolor flow cytometric analysis of CD103 Integrin alphaE expression on Mouse lymph node cells. C57BL/6 Mouse Lymph node cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and BD Horizon RB705 Hamster Anti-Mouse CD103 Integrin alphaE antibody Cat. No. 570571/570659; Right Plot at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expressed levels of CD103 Integrin alphaE versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymph node cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD103 Integrin alphaE expression on Mouse lymph node cells. C57BL/6 Mouse Lymph node cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and BD Horizon RB705 Hamster Anti-Mouse CD103 Integrin alphaE antibody Cat. No. 570571/570659; Right Plot at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expressed levels of CD103 Integrin alphaE versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymph node cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD103 Integrin alphaE expression on Mouse lymph node cells. C57BL/6 Mouse Lymph node cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and BD Horizon RB705 Hamster Anti-Mouse CD103 Integrin alphaE antibody Cat. No. 570571/570659; Right Plot at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expressed levels of CD103 Integrin alphaE versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymph node cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.