BD HorizonTM RB705 Hamster Anti-Mouse CD3e

Description

Multicolor flow cytometric analysis of CD3e expression on viable Mouse splenic leucocytes. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 . The cells were then stained with FITC Rat Anti-Mouse CD4 Cat. No. 553047 and FITC Rat Anti-Mouse CD8a Cat. No. 553030 antibodies and with either no BD Horizon RB705 conjugated antibody Autofluorescence Control; Left Plot or BD Horizon RB705 Hamster Anti-Mouse CD3e antibody Cat. No. 570560/570648; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD3e or cellular Autofluorescence versus CD4 and CD8a was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD3e expression on viable Mouse splenic leucocytes. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 . The cells were then stained with FITC Rat Anti-Mouse CD4 Cat. No. 553047 and FITC Rat Anti-Mouse CD8a Cat. No. 553030 antibodies and with either no BD Horizon RB705 conjugated antibody Autofluorescence Control; Left Plot or BD Horizon RB705 Hamster Anti-Mouse CD3e antibody Cat. No. 570560/570648; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD3e or cellular Autofluorescence versus CD4 and CD8a was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD3e expression on viable Mouse splenic leucocytes. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 . The cells were then stained with FITC Rat Anti-Mouse CD4 Cat. No. 553047 and FITC Rat Anti-Mouse CD8a Cat. No. 553030 antibodies and with either no BD Horizon RB705 conjugated antibody Autofluorescence Control; Left Plot or BD Horizon RB705 Hamster Anti-Mouse CD3e antibody Cat. No. 570560/570648; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD3e or cellular Autofluorescence versus CD4 and CD8a was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD3e expression on viable Mouse splenic leucocytes. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 . The cells were then stained with FITC Rat Anti-Mouse CD4 Cat. No. 553047 and FITC Rat Anti-Mouse CD8a Cat. No. 553030 antibodies and with either no BD Horizon RB705 conjugated antibody Autofluorescence Control; Left Plot or BD Horizon RB705 Hamster Anti-Mouse CD3e antibody Cat. No. 570560/570648; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD3e or cellular Autofluorescence versus CD4 and CD8a was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD3e expression on viable Mouse splenic leucocytes. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 . The cells were then stained with FITC Rat Anti-Mouse CD4 Cat. No. 553047 and FITC Rat Anti-Mouse CD8a Cat. No. 553030 antibodies and with either no BD Horizon RB705 conjugated antibody Autofluorescence Control; Left Plot or BD Horizon RB705 Hamster Anti-Mouse CD3e antibody Cat. No. 570560/570648; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD3e or cellular Autofluorescence versus CD4 and CD8a was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD3e expression on viable Mouse splenic leucocytes. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 . The cells were then stained with FITC Rat Anti-Mouse CD4 Cat. No. 553047 and FITC Rat Anti-Mouse CD8a Cat. No. 553030 antibodies and with either no BD Horizon RB705 conjugated antibody Autofluorescence Control; Left Plot or BD Horizon RB705 Hamster Anti-Mouse CD3e antibody Cat. No. 570560/570648; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD3e or cellular Autofluorescence versus CD4 and CD8a was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.