BD HorizonTM RB705 Hamster Anti-Mouse CD80 (B7-1)

Description

Flow cytometric analysis of CD80 B7-1 expression on viable activated versus resting Mouse splenic leukocytes. Freshly isolated dashed line histogram or 72-hour lipopolysaccharide LPS -stimulated BALB/c splenic leukocytes solid line histogram were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 . The cells were then stained with BD Horizon RB705 Hamster Anti-Mouse CD80 B7-1 antibody Cat. No. 570549/570553 at 0.25 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The histograms showing CD80 B7-1 expression were derived from gated events with the forward and side-light scatter characteristics of viable DAPI negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD80 B7-1 expression on viable activated versus resting Mouse splenic leukocytes. Freshly isolated dashed line histogram or 72-hour lipopolysaccharide LPS -stimulated BALB/c splenic leukocytes solid line histogram were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 . The cells were then stained with BD Horizon RB705 Hamster Anti-Mouse CD80 B7-1 antibody Cat. No. 570549/570553 at 0.25 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The histograms showing CD80 B7-1 expression were derived from gated events with the forward and side-light scatter characteristics of viable DAPI negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD80 B7-1 expression on viable activated versus resting Mouse splenic leukocytes. Freshly isolated dashed line histogram or 72-hour lipopolysaccharide LPS -stimulated BALB/c splenic leukocytes solid line histogram were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 . The cells were then stained with BD Horizon RB705 Hamster Anti-Mouse CD80 B7-1 antibody Cat. No. 570549/570553 at 0.25 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The histograms showing CD80 B7-1 expression were derived from gated events with the forward and side-light scatter characteristics of viable DAPI negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.