BD HorizonTM RB705 Mouse Anti-GATA3

Description

Flow cytometric analysis of GATA3 expression in Human Jurkat cells. Cells from the Human Ramos Burkitts lymphoma, ATCC CRL-1596; dashed line histogram and Jurkat Acute T cell leukemia, ATCC TIB-152; solid line histogram cell lines were fixed 10 min, 37oC with pre-warmed BD Cytofix Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with BD Horizon RB705 Mouse Anti-GATA3 antibody Cat. No. 570618/570706 . The fluorescence histograms showing GATA3 expression were derived from gated events with the forward and side light-scatter characteristics of intact lymphoid cells. Flow cytometry and data analysis were performed using a LSRFortessa X-20 Flow Cytometer System and FlowJo Software. Flow cytometric analysis of GATA3 expression in Mouse D10.G4.1 cells. Cells from the Mouse 2D6 Ahn et al, 1998; dashed line histogram and D10.G4.1 T Helper ATCC TIB-224; solid line histogram cell lines were fixed 10 min, 37oC with pre-warmed BD Cytofix Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with BD Horizon RB705 Mouse Anti-GATA3 antibody Cat. No. 570618/570706 . The fluorescence histograms showing GATA3 expression were derived from gated events with the forward and side light-scatter characteristics of intact lymphoid cells. Flow cytometry and data analysis were performed using a LSRFortessa X-20 Flow Cytometer System and FlowJo Software. Flow cytometric analysis of GATA3 expression in Human Jurkat cells. Cells from the Human Ramos Burkitts lymphoma, ATCC CRL-1596; dashed line histogram and Jurkat Acute T cell leukemia, ATCC TIB-152; solid line histogram cell lines were fixed 10 min, 37oC with pre-warmed BD Cytofix Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with BD Horizon RB705 Mouse Anti-GATA3 antibody Cat. No. 570618/570706 . The fluorescence histograms showing GATA3 expression were derived from gated events with the forward and side light-scatter characteristics of intact lymphoid cells. Flow cytometry and data analysis were performed using a LSRFortessa X-20 Flow Cytometer System and FlowJo Software. Show More Flow cytometric analysis of GATA3 expression in Mouse D10.G4.1 cells. Cells from the Mouse 2D6 Ahn et al, 1998; dashed line histogram and D10.G4.1 T Helper ATCC TIB-224; solid line histogram cell lines were fixed 10 min, 37oC with pre-warmed BD Cytofix Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with BD Horizon RB705 Mouse Anti-GATA3 antibody Cat. No. 570618/570706 . The fluorescence histograms showing GATA3 expression were derived from gated events with the forward and side light-scatter characteristics of intact lymphoid cells. Flow cytometry and data analysis were performed using a LSRFortessa X-20 Flow Cytometer System and FlowJo Software. Show More Flow cytometric analysis of GATA3 expression in Human Jurkat cells. Cells from the Human Ramos Burkitts lymphoma, ATCC CRL-1596; dashed line histogram and Jurkat Acute T cell leukemia, ATCC TIB-152; solid line histogram cell lines were fixed 10 min, 37oC with pre-warmed BD Cytofix Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with BD Horizon RB705 Mouse Anti-GATA3 antibody Cat. No. 570618/570706 . The fluorescence histograms showing GATA3 expression were derived from gated events with the forward and side light-scatter characteristics of intact lymphoid cells. Flow cytometry and data analysis were performed using a LSRFortessa X-20 Flow Cytometer System and FlowJo Software. Flow cytometric analysis of GATA3 expression in Mouse D10.G4.1 cells. Cells from the Mouse 2D6 Ahn et al, 1998; dashed line histogram and D10.G4.1 T Helper ATCC TIB-224; solid line histogram cell lines were fixed 10 min, 37oC with pre-warmed BD Cytofix Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with BD Horizon RB705 Mouse Anti-GATA3 antibody Cat. No. 570618/570706 . The fluorescence histograms showing GATA3 expression were derived from gated events with the forward and side light-scatter characteristics of intact lymphoid cells. Flow cytometry and data analysis were performed using a LSRFortessa X-20 Flow Cytometer System and FlowJo Software.