BD HorizonTM RB705 Mouse Anti-Human CD137 (4-1BB)

Description

Flow cytometric analysis of CD137 4-1BB expression on viable stimulated Human peripheral blood lymphocytes. Concanavalin A Con-A -stimulated 3 days peripheral blood mononuclear cells PBMC were stained with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; dashed line histogram or BD Horizon RB705 Mouse Anti-Human CD137 4-1BB antibody Cat. No. 570577/570665; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing the expression of CD137 4-1BB or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative activated lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Flow cytometric analysis of CD137 4-1BB expression on viable stimulated Human peripheral blood lymphocytes. Concanavalin A Con-A -stimulated 3 days peripheral blood mononuclear cells PBMC were stained with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; dashed line histogram or BD Horizon RB705 Mouse Anti-Human CD137 4-1BB antibody Cat. No. 570577/570665; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing the expression of CD137 4-1BB or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative activated lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Flow cytometric analysis of CD137 4-1BB expression on viable stimulated Human peripheral blood lymphocytes. Concanavalin A Con-A -stimulated 3 days peripheral blood mononuclear cells PBMC were stained with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; dashed line histogram or BD Horizon RB705 Mouse Anti-Human CD137 4-1BB antibody Cat. No. 570577/570665; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing the expression of CD137 4-1BB or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative activated lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.