BD HorizonTM RB705 Mouse Anti-Human CD152 (CTLA-4)

Description

Multicolor flow cytometric analysis of CD152 CTLA-4 expression on activated Human peripheral blood lymphocytes. Phytohemagglutinin PHA -activated 3 days Human peripheral blood mononuclear cells PBMC were preincubated with Human BD Fc Block Cat. No. 564219/564220 . The cells were then stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333/561808 and with either BD Horizon RB705 Mouse IgG2a, kappa Isotype Control Cat. No. 570254; Left Plot or BD Horizon RB705 Mouse Anti-Human CD152 CTLA-4 antibody Cat. No. 570590/570678; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD152 CTLA-4 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Multicolor flow cytometric analysis of CD152 CTLA-4 expression on activated Human peripheral blood lymphocytes. Phytohemagglutinin PHA -activated 3 days Human peripheral blood mononuclear cells PBMC were preincubated with Human BD Fc Block Cat. No. 564219/564220 . The cells were then stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333/561808 and with either BD Horizon RB705 Mouse IgG2a, kappa Isotype Control Cat. No. 570254; Left Plot or BD Horizon RB705 Mouse Anti-Human CD152 CTLA-4 antibody Cat. No. 570590/570678; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD152 CTLA-4 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Multicolor flow cytometric analysis of CD152 CTLA-4 expression on activated Human peripheral blood lymphocytes. Phytohemagglutinin PHA -activated 3 days Human peripheral blood mononuclear cells PBMC were preincubated with Human BD Fc Block Cat. No. 564219/564220 . The cells were then stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333/561808 and with either BD Horizon RB705 Mouse IgG2a, kappa Isotype Control Cat. No. 570254; Left Plot or BD Horizon RB705 Mouse Anti-Human CD152 CTLA-4 antibody Cat. No. 570590/570678; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD152 CTLA-4 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Show More Multicolor flow cytometric analysis of CD152 CTLA-4 expression on activated Human peripheral blood lymphocytes. Phytohemagglutinin PHA -activated 3 days Human peripheral blood mononuclear cells PBMC were preincubated with Human BD Fc Block Cat. No. 564219/564220 . The cells were then stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333/561808 and with either BD Horizon RB705 Mouse IgG2a, kappa Isotype Control Cat. No. 570254; Left Plot or BD Horizon RB705 Mouse Anti-Human CD152 CTLA-4 antibody Cat. No. 570590/570678; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD152 CTLA-4 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Show More Multicolor flow cytometric analysis of CD152 CTLA-4 expression on activated Human peripheral blood lymphocytes. Phytohemagglutinin PHA -activated 3 days Human peripheral blood mononuclear cells PBMC were preincubated with Human BD Fc Block Cat. No. 564219/564220 . The cells were then stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333/561808 and with either BD Horizon RB705 Mouse IgG2a, kappa Isotype Control Cat. No. 570254; Left Plot or BD Horizon RB705 Mouse Anti-Human CD152 CTLA-4 antibody Cat. No. 570590/570678; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD152 CTLA-4 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Multicolor flow cytometric analysis of CD152 CTLA-4 expression on activated Human peripheral blood lymphocytes. Phytohemagglutinin PHA -activated 3 days Human peripheral blood mononuclear cells PBMC were preincubated with Human BD Fc Block Cat. No. 564219/564220 . The cells were then stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333/561808 and with either BD Horizon RB705 Mouse IgG2a, kappa Isotype Control Cat. No. 570254; Left Plot or BD Horizon RB705 Mouse Anti-Human CD152 CTLA-4 antibody Cat. No. 570590/570678; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD152 CTLA-4 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software.