
570660 · 25 Tests
BDB Bioscience · Cat: 570660
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Multiparameter flow cytometric analysis of CD20 expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed and preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564220 . The leukocytes were stained with either BD Horizon RB705 Mouse IgG2b, kappa Isotype Control Cat. No. 570273; Left Plot or BD Horizon RB705 Mouse Anti-Human CD20 antibody Cat. No. 570572/570660; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD20 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer and FlowJo Software. Multiparameter flow cytometric analysis of CD20 expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed and preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564220 . The leukocytes were stained with either BD Horizon RB705 Mouse IgG2b, kappa Isotype Control Cat. No. 570273; Left Plot or BD Horizon RB705 Mouse Anti-Human CD20 antibody Cat. No. 570572/570660; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD20 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer and FlowJo Software. Multiparameter flow cytometric analysis of CD20 expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed and preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564220 . The leukocytes were stained with either BD Horizon RB705 Mouse IgG2b, kappa Isotype Control Cat. No. 570273; Left Plot or BD Horizon RB705 Mouse Anti-Human CD20 antibody Cat. No. 570572/570660; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD20 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer and FlowJo Software. Show More Multiparameter flow cytometric analysis of CD20 expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed and preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564220 . The leukocytes were stained with either BD Horizon RB705 Mouse IgG2b, kappa Isotype Control Cat. No. 570273; Left Plot or BD Horizon RB705 Mouse Anti-Human CD20 antibody Cat. No. 570572/570660; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD20 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer and FlowJo Software. Show More Multiparameter flow cytometric analysis of CD20 expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed and preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564220 . The leukocytes were stained with either BD Horizon RB705 Mouse IgG2b, kappa Isotype Control Cat. No. 570273; Left Plot or BD Horizon RB705 Mouse Anti-Human CD20 antibody Cat. No. 570572/570660; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD20 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer and FlowJo Software. Multiparameter flow cytometric analysis of CD20 expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed and preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564220 . The leukocytes were stained with either BD Horizon RB705 Mouse IgG2b, kappa Isotype Control Cat. No. 570273; Left Plot or BD Horizon RB705 Mouse Anti-Human CD20 antibody Cat. No. 570572/570660; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD20 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer and FlowJo Software.