BD HorizonTM RB705 Mouse Anti-Human CD25 (IL-2 Receptor alpha)

Description

Multicolor flow cytometric analysis for CD25 IL-2 Receptor alpha on unstimulated Human peripheral blood lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD4 antibody Cat. No. 555349/561840 and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; Left Plot or BD Horizon RB705 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 570249/570250; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer System and FlowJo software. Flow cytometric analysis for CD25 IL-2 Receptor alpha on stimulated Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin. The cells were then stained with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RB705 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer System and FlowJo software. Multicolor flow cytometric analysis for CD25 IL-2 Receptor alpha on unstimulated Human peripheral blood lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD4 antibody Cat. No. 555349/561840 and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; Left Plot or BD Horizon RB705 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 570249/570250; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer System and FlowJo software. Show More Flow cytometric analysis for CD25 IL-2 Receptor alpha on stimulated Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin. The cells were then stained with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RB705 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer System and FlowJo software. Show More Multicolor flow cytometric analysis for CD25 IL-2 Receptor alpha on unstimulated Human peripheral blood lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD4 antibody Cat. No. 555349/561840 and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; Left Plot or BD Horizon RB705 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 570249/570250; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer System and FlowJo software. Flow cytometric analysis for CD25 IL-2 Receptor alpha on stimulated Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin. The cells were then stained with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RB705 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer System and FlowJo software.