BD HorizonTM RB705 Mouse Anti-Human CD279 (PD-1)

Description

Two-color flow cytometric analysis of CD279 PD-1 expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426 and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; Left Plot or BD Horizon RB705 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 570246/570245; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD279 PD-1 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo software. Two-color flow cytometric analysis of CD279 PD-1 expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426 and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; Left Plot or BD Horizon RB705 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 570246/570245; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD279 PD-1 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo software. Two-color flow cytometric analysis of CD279 PD-1 expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426 and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; Left Plot or BD Horizon RB705 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 570246/570245; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD279 PD-1 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo software. Show More Two-color flow cytometric analysis of CD279 PD-1 expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426 and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; Left Plot or BD Horizon RB705 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 570246/570245; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD279 PD-1 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo software. Show More Two-color flow cytometric analysis of CD279 PD-1 expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426 and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; Left Plot or BD Horizon RB705 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 570246/570245; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD279 PD-1 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo software. Two-color flow cytometric analysis of CD279 PD-1 expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426 and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; Left Plot or BD Horizon RB705 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 570246/570245; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD279 PD-1 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo software.