
570732 · 25 Tests
BDB Bioscience · Cat: 570732
Reach 3+ verified distributors
Manufacturer product page
bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow cytometric analysis of CD68 expression by Human peripheral blood monocytes. Human peripheral blood mononuclear cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . After washing with BD Perm/Wash Buffer Cat. No. 554723 , the cells were stained in BD Perm/Wash Buffer with either BD Horizon RB705 Mouse IgG2b, kappa Isotype Control Cat. No. 570273; dashed line histogram or BD Horizon RB705 Mouse Anti-Human CD68 antibody Cat. No. 570646/570732; solid line histogram . The fluorescence histogram showing CD68 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD68 expression by Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . After washing with BD Perm/Wash Buffer Cat. No. 554723 , the cells were stained in BD Perm/Wash Buffer with either BD Horizon RB705 Mouse IgG2b, kappa Isotype Control Cat. No. 570273; Left Plot or BD Horizon RB705 Mouse Anti-Human CD68 antibody Cat. No. 570646/570732; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD68 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD68 expression by Human peripheral blood monocytes. Human peripheral blood mononuclear cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . After washing with BD Perm/Wash Buffer Cat. No. 554723 , the cells were stained in BD Perm/Wash Buffer with either BD Horizon RB705 Mouse IgG2b, kappa Isotype Control Cat. No. 570273; dashed line histogram or BD Horizon RB705 Mouse Anti-Human CD68 antibody Cat. No. 570646/570732; solid line histogram . The fluorescence histogram showing CD68 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of CD68 expression by Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . After washing with BD Perm/Wash Buffer Cat. No. 554723 , the cells were stained in BD Perm/Wash Buffer with either BD Horizon RB705 Mouse IgG2b, kappa Isotype Control Cat. No. 570273; Left Plot or BD Horizon RB705 Mouse Anti-Human CD68 antibody Cat. No. 570646/570732; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD68 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of CD68 expression by Human peripheral blood monocytes. Human peripheral blood mononuclear cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . After washing with BD Perm/Wash Buffer Cat. No. 554723 , the cells were stained in BD Perm/Wash Buffer with either BD Horizon RB705 Mouse IgG2b, kappa Isotype Control Cat. No. 570273; dashed line histogram or BD Horizon RB705 Mouse Anti-Human CD68 antibody Cat. No. 570646/570732; solid line histogram . The fluorescence histogram showing CD68 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD68 expression by Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . After washing with BD Perm/Wash Buffer Cat. No. 554723 , the cells were stained in BD Perm/Wash Buffer with either BD Horizon RB705 Mouse IgG2b, kappa Isotype Control Cat. No. 570273; Left Plot or BD Horizon RB705 Mouse Anti-Human CD68 antibody Cat. No. 570646/570732; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD68 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.