BD HorizonTM RB705 Mouse Anti-Human CD86 (B7-2)

Description

Multiparameter flow cytometric analysis of CD86 B7-2 expression on Human peripheral blood leukocyte populations. Fresh whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and then stained with either BD Horizon RB705 Mouse IgG2b, kappa Isotype Control Cat. No. 570273; Left Plot or BD Horizon RB705 Mouse Anti-Human CD86 B7-2 antibody Cat. No. 570615/570703; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD86 B7-2 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD86 B7-2 expression on Human peripheral blood leukocyte populations. Fresh whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and then stained with either BD Horizon RB705 Mouse IgG2b, kappa Isotype Control Cat. No. 570273; Left Plot or BD Horizon RB705 Mouse Anti-Human CD86 B7-2 antibody Cat. No. 570615/570703; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD86 B7-2 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD86 B7-2 expression on Human peripheral blood leukocyte populations. Fresh whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and then stained with either BD Horizon RB705 Mouse IgG2b, kappa Isotype Control Cat. No. 570273; Left Plot or BD Horizon RB705 Mouse Anti-Human CD86 B7-2 antibody Cat. No. 570615/570703; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD86 B7-2 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of CD86 B7-2 expression on Human peripheral blood leukocyte populations. Fresh whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and then stained with either BD Horizon RB705 Mouse IgG2b, kappa Isotype Control Cat. No. 570273; Left Plot or BD Horizon RB705 Mouse Anti-Human CD86 B7-2 antibody Cat. No. 570615/570703; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD86 B7-2 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of CD86 B7-2 expression on Human peripheral blood leukocyte populations. Fresh whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and then stained with either BD Horizon RB705 Mouse IgG2b, kappa Isotype Control Cat. No. 570273; Left Plot or BD Horizon RB705 Mouse Anti-Human CD86 B7-2 antibody Cat. No. 570615/570703; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD86 B7-2 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD86 B7-2 expression on Human peripheral blood leukocyte populations. Fresh whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and then stained with either BD Horizon RB705 Mouse IgG2b, kappa Isotype Control Cat. No. 570273; Left Plot or BD Horizon RB705 Mouse Anti-Human CD86 B7-2 antibody Cat. No. 570615/570703; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD86 B7-2 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.