BD HorizonTM RB705 Mouse Anti-Human CD90 (Thy-1)

Description

Multicolor flow cytometric analysis of CD90 Thy-1 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stained with FITC Mouse Anti-Human CD34 Cat. No. 555821/560942 and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; Left Plot or BD Horizon RB705 Mouse Anti-Human CD90 Thy-1 antibody Cat. No. 570580/570668; Right Plot . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD90 Thy-1 or Ig Isotype control staining versus CD34 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Flow cytometric analysis of CD90 Thy-1 expression on Human HEL cells. Cells from the Human HEL Human erythroleukemia, ATCC TIB-180 cell line were stained with BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; dashed line histogram or BD Horizon RB705 Mouse Anti-Human CD90 Thy-1 antibody Cat. No. 570580/570668; solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD90 Thy-1 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Multicolor flow cytometric analysis of CD90 Thy-1 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stained with FITC Mouse Anti-Human CD34 Cat. No. 555821/560942 and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; Left Plot or BD Horizon RB705 Mouse Anti-Human CD90 Thy-1 antibody Cat. No. 570580/570668; Right Plot . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD90 Thy-1 or Ig Isotype control staining versus CD34 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Flow cytometric analysis of CD90 Thy-1 expression on Human HEL cells. Cells from the Human HEL Human erythroleukemia, ATCC TIB-180 cell line were stained with BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; dashed line histogram or BD Horizon RB705 Mouse Anti-Human CD90 Thy-1 antibody Cat. No. 570580/570668; solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD90 Thy-1 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Show More Multicolor flow cytometric analysis of CD90 Thy-1 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stained with FITC Mouse Anti-Human CD34 Cat. No. 555821/560942 and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; Left Plot or BD Horizon RB705 Mouse Anti-Human CD90 Thy-1 antibody Cat. No. 570580/570668; Right Plot . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD90 Thy-1 or Ig Isotype control staining versus CD34 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Flow cytometric analysis of CD90 Thy-1 expression on Human HEL cells. Cells from the Human HEL Human erythroleukemia, ATCC TIB-180 cell line were stained with BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; dashed line histogram or BD Horizon RB705 Mouse Anti-Human CD90 Thy-1 antibody Cat. No. 570580/570668; solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD90 Thy-1 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software.