BD HorizonTM RB705 Mouse Anti-Human IFN-gamma

Description

Two-color flow cytometric analysis of IFN-gamma expression in stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 for staining with APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; Left Plot or BD Horizon RB705 Mouse Anti-Human IFN-gamma antibody Cat. No. 570247/570248; Right Plot .The bivariate pseudocolor density plot showing the correlated expression of IFN-gamma or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Two-color flow cytometric analysis of IFN-gamma expression in stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 for staining with APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; Left Plot or BD Horizon RB705 Mouse Anti-Human IFN-gamma antibody Cat. No. 570247/570248; Right Plot .The bivariate pseudocolor density plot showing the correlated expression of IFN-gamma or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Two-color flow cytometric analysis of IFN-gamma expression in stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 for staining with APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; Left Plot or BD Horizon RB705 Mouse Anti-Human IFN-gamma antibody Cat. No. 570247/570248; Right Plot .The bivariate pseudocolor density plot showing the correlated expression of IFN-gamma or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Show More Two-color flow cytometric analysis of IFN-gamma expression in stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 for staining with APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; Left Plot or BD Horizon RB705 Mouse Anti-Human IFN-gamma antibody Cat. No. 570247/570248; Right Plot .The bivariate pseudocolor density plot showing the correlated expression of IFN-gamma or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Show More Two-color flow cytometric analysis of IFN-gamma expression in stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 for staining with APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; Left Plot or BD Horizon RB705 Mouse Anti-Human IFN-gamma antibody Cat. No. 570247/570248; Right Plot .The bivariate pseudocolor density plot showing the correlated expression of IFN-gamma or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Two-color flow cytometric analysis of IFN-gamma expression in stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 for staining with APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; Left Plot or BD Horizon RB705 Mouse Anti-Human IFN-gamma antibody Cat. No. 570247/570248; Right Plot .The bivariate pseudocolor density plot showing the correlated expression of IFN-gamma or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software.