BD HorizonTM RB705 Mouse Anti-Human IL-3 Receptor alpha (CD123)

Description

Multiparameter flow cytometric analysis of IL-3 Receptor alpha CD123 expression on Human peripheral blood leukocyte populations. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD45RO Cat No. 562641/562649; Bottom Plots and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; Left Plots or BD Horizon RB705 Mouse Anti-Human IL-3 Receptor alpha CD123 antibody Cat. No. 570582/570670; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat No. 349202 . Top Plots: Bivariate pseudocolor density plots showing the correlated expression of IL-3 Receptor alpha CD123 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Bottom Plots: Bivariate pseudocolor density plots showing the correlated expression of IL-3 Receptor alpha CD123 or Ig Isotype control staining versus CD45RO were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of IL-3 Receptor alpha CD123 expression on Human peripheral blood leukocyte populations. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD45RO Cat No. 562641/562649; Bottom Plots and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; Left Plots or BD Horizon RB705 Mouse Anti-Human IL-3 Receptor alpha CD123 antibody Cat. No. 570582/570670; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat No. 349202 . Top Plots: Bivariate pseudocolor density plots showing the correlated expression of IL-3 Receptor alpha CD123 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Bottom Plots: Bivariate pseudocolor density plots showing the correlated expression of IL-3 Receptor alpha CD123 or Ig Isotype control staining versus CD45RO were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of IL-3 Receptor alpha CD123 expression on Human peripheral blood leukocyte populations. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD45RO Cat No. 562641/562649; Bottom Plots and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; Left Plots or BD Horizon RB705 Mouse Anti-Human IL-3 Receptor alpha CD123 antibody Cat. No. 570582/570670; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat No. 349202 . Top Plots: Bivariate pseudocolor density plots showing the correlated expression of IL-3 Receptor alpha CD123 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Bottom Plots: Bivariate pseudocolor density plots showing the correlated expression of IL-3 Receptor alpha CD123 or Ig Isotype control staining versus CD45RO were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.