
571587 · 25 ug
BDB Bioscience · Cat: 571587
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Multiparameter flow cytometric analysis of TCR Cbeta2 expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD OptiBuild RB545 Mouse Anti-Human TCR Cbeta1 antibody Cat. No. 756564 and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; Left Plot or BD Horizon RB705 Mouse Anti-Human TCR Cbeta2 antibody Cat. No. 571545/571587; Right Plot at 0.5 mug/test. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of TCR Cbeta2 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of TCR Cbeta2 expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD OptiBuild RB545 Mouse Anti-Human TCR Cbeta1 antibody Cat. No. 756564 and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; Left Plot or BD Horizon RB705 Mouse Anti-Human TCR Cbeta2 antibody Cat. No. 571545/571587; Right Plot at 0.5 mug/test. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of TCR Cbeta2 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of TCR Cbeta2 expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD OptiBuild RB545 Mouse Anti-Human TCR Cbeta1 antibody Cat. No. 756564 and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; Left Plot or BD Horizon RB705 Mouse Anti-Human TCR Cbeta2 antibody Cat. No. 571545/571587; Right Plot at 0.5 mug/test. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of TCR Cbeta2 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of TCR Cbeta2 expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD OptiBuild RB545 Mouse Anti-Human TCR Cbeta1 antibody Cat. No. 756564 and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; Left Plot or BD Horizon RB705 Mouse Anti-Human TCR Cbeta2 antibody Cat. No. 571545/571587; Right Plot at 0.5 mug/test. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of TCR Cbeta2 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of TCR Cbeta2 expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD OptiBuild RB545 Mouse Anti-Human TCR Cbeta1 antibody Cat. No. 756564 and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; Left Plot or BD Horizon RB705 Mouse Anti-Human TCR Cbeta2 antibody Cat. No. 571545/571587; Right Plot at 0.5 mug/test. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of TCR Cbeta2 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of TCR Cbeta2 expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD OptiBuild RB545 Mouse Anti-Human TCR Cbeta1 antibody Cat. No. 756564 and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; Left Plot or BD Horizon RB705 Mouse Anti-Human TCR Cbeta2 antibody Cat. No. 571545/571587; Right Plot at 0.5 mug/test. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of TCR Cbeta2 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.