BD HorizonTM RB705 Mouse Anti-Human TCRalphabeta

Description

Multicolor flow cytometric analysis of TCRalphabeta expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD19 antibody Cat. No. 562440/562441; Top Plots , APC Mouse Anti-Human CD3 antibody Cat. No. 561811/561810; Bottom Plots and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat No. 570261; Left Plots or BD Horizon RB705 Mouse Anti-Human TCRalphabeta antibody Cat. No. 570614/570702; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Top Plots: Bivariate pseudocolor density plots showing the correlated expression of TCRalphabeta or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Bottom Plots: Bivariate pseudocolor density plots showing the correlated expression of TCRalphabeta or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Multicolor flow cytometric analysis of TCRalphabeta expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD19 antibody Cat. No. 562440/562441; Top Plots , APC Mouse Anti-Human CD3 antibody Cat. No. 561811/561810; Bottom Plots and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat No. 570261; Left Plots or BD Horizon RB705 Mouse Anti-Human TCRalphabeta antibody Cat. No. 570614/570702; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Top Plots: Bivariate pseudocolor density plots showing the correlated expression of TCRalphabeta or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Bottom Plots: Bivariate pseudocolor density plots showing the correlated expression of TCRalphabeta or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Show More Multicolor flow cytometric analysis of TCRalphabeta expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD19 antibody Cat. No. 562440/562441; Top Plots , APC Mouse Anti-Human CD3 antibody Cat. No. 561811/561810; Bottom Plots and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat No. 570261; Left Plots or BD Horizon RB705 Mouse Anti-Human TCRalphabeta antibody Cat. No. 570614/570702; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Top Plots: Bivariate pseudocolor density plots showing the correlated expression of TCRalphabeta or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Bottom Plots: Bivariate pseudocolor density plots showing the correlated expression of TCRalphabeta or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes.