BD HorizonTM RB705 Mouse Anti-Human TIM-3 (CD366)

Description

Multiparameter flow cytometric analysis of TIM-3 CD366 expression on Human peripheral blood leukocyte populations. Human whole blood was stained with FITC Mouse Anti-Human CD56 NCAM-1 antibody Cat. No. 562794; Lower Plots and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; Left Plots or BD Horizon RB705 Mouse Anti-Human TIM-3 CD366 antibody Cat. No. 570584/570672; Right Plots . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Top Plots - The bivariate pseudocolor density plot showing TIM-3 CD366 expression or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leukocyte populations. Lower Plots - The bivariate pseudocolor density plot showing the correlated expression of TIM-3 CD366 or Ig Isotype control staining versus CD56 NCAM-1 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of TIM-3 CD366 expression on Human peripheral blood leukocyte populations. Human whole blood was stained with FITC Mouse Anti-Human CD56 NCAM-1 antibody Cat. No. 562794; Lower Plots and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; Left Plots or BD Horizon RB705 Mouse Anti-Human TIM-3 CD366 antibody Cat. No. 570584/570672; Right Plots . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Top Plots - The bivariate pseudocolor density plot showing TIM-3 CD366 expression or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leukocyte populations. Lower Plots - The bivariate pseudocolor density plot showing the correlated expression of TIM-3 CD366 or Ig Isotype control staining versus CD56 NCAM-1 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of TIM-3 CD366 expression on Human peripheral blood leukocyte populations. Human whole blood was stained with FITC Mouse Anti-Human CD56 NCAM-1 antibody Cat. No. 562794; Lower Plots and with either BD Horizon RB705 Mouse IgG1, kappa Isotype Control Cat. No. 570261; Left Plots or BD Horizon RB705 Mouse Anti-Human TIM-3 CD366 antibody Cat. No. 570584/570672; Right Plots . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Top Plots - The bivariate pseudocolor density plot showing TIM-3 CD366 expression or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leukocyte populations. Lower Plots - The bivariate pseudocolor density plot showing the correlated expression of TIM-3 CD366 or Ig Isotype control staining versus CD56 NCAM-1 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software.