BD HorizonTM RB705 Mouse Anti-Mouse CD161b,c (NK-1.1)

Description

Multicolor flow cytometric analysis of CD161b,c NK-1.1 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 and Purified Armenian Hamster Anti-Mouse FcgammaRIV CD16-2 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and with either BD Horizon RB705 Mouse IgG2a, kappa Isotype Control Cat. No. 570254, Left Plot or BD Horizon RB705 Mouse Anti-Mouse CD161b,c NK-1.1 antibody Cat. No. 570630/570717; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD161b,c NK-1.1 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD161b,c NK-1.1 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 and Purified Armenian Hamster Anti-Mouse FcgammaRIV CD16-2 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and with either BD Horizon RB705 Mouse IgG2a, kappa Isotype Control Cat. No. 570254, Left Plot or BD Horizon RB705 Mouse Anti-Mouse CD161b,c NK-1.1 antibody Cat. No. 570630/570717; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD161b,c NK-1.1 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD161b,c NK-1.1 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 and Purified Armenian Hamster Anti-Mouse FcgammaRIV CD16-2 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and with either BD Horizon RB705 Mouse IgG2a, kappa Isotype Control Cat. No. 570254, Left Plot or BD Horizon RB705 Mouse Anti-Mouse CD161b,c NK-1.1 antibody Cat. No. 570630/570717; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD161b,c NK-1.1 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD161b,c NK-1.1 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 and Purified Armenian Hamster Anti-Mouse FcgammaRIV CD16-2 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and with either BD Horizon RB705 Mouse IgG2a, kappa Isotype Control Cat. No. 570254, Left Plot or BD Horizon RB705 Mouse Anti-Mouse CD161b,c NK-1.1 antibody Cat. No. 570630/570717; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD161b,c NK-1.1 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD161b,c NK-1.1 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 and Purified Armenian Hamster Anti-Mouse FcgammaRIV CD16-2 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and with either BD Horizon RB705 Mouse IgG2a, kappa Isotype Control Cat. No. 570254, Left Plot or BD Horizon RB705 Mouse Anti-Mouse CD161b,c NK-1.1 antibody Cat. No. 570630/570717; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD161b,c NK-1.1 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD161b,c NK-1.1 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 and Purified Armenian Hamster Anti-Mouse FcgammaRIV CD16-2 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and with either BD Horizon RB705 Mouse IgG2a, kappa Isotype Control Cat. No. 570254, Left Plot or BD Horizon RB705 Mouse Anti-Mouse CD161b,c NK-1.1 antibody Cat. No. 570630/570717; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD161b,c NK-1.1 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.