BD HorizonTM RB705 Rat Anti-Mouse CD117 (c-Kit)

Description

Multicolor flow cytometric analysis of CD117 c-Kit expression on Mouse bone marrow cells. BALB/c Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD45R/B220 Cat. No. 553090/561878 and with either BD Horizon RB705 Rat IgG2b, kappa Isotype Control Cat. No. 570263; Left Plot or BD Horizon RB705 Rat Anti-Mouse CD117 c-Kit antibody Cat. No. 570567/570655; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of CD117 c-Kit or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD117 c-Kit expression on Mouse bone marrow cells. BALB/c Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD45R/B220 Cat. No. 553090/561878 and with either BD Horizon RB705 Rat IgG2b, kappa Isotype Control Cat. No. 570263; Left Plot or BD Horizon RB705 Rat Anti-Mouse CD117 c-Kit antibody Cat. No. 570567/570655; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of CD117 c-Kit or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD117 c-Kit expression on Mouse bone marrow cells. BALB/c Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD45R/B220 Cat. No. 553090/561878 and with either BD Horizon RB705 Rat IgG2b, kappa Isotype Control Cat. No. 570263; Left Plot or BD Horizon RB705 Rat Anti-Mouse CD117 c-Kit antibody Cat. No. 570567/570655; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of CD117 c-Kit or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD117 c-Kit expression on Mouse bone marrow cells. BALB/c Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD45R/B220 Cat. No. 553090/561878 and with either BD Horizon RB705 Rat IgG2b, kappa Isotype Control Cat. No. 570263; Left Plot or BD Horizon RB705 Rat Anti-Mouse CD117 c-Kit antibody Cat. No. 570567/570655; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of CD117 c-Kit or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD117 c-Kit expression on Mouse bone marrow cells. BALB/c Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD45R/B220 Cat. No. 553090/561878 and with either BD Horizon RB705 Rat IgG2b, kappa Isotype Control Cat. No. 570263; Left Plot or BD Horizon RB705 Rat Anti-Mouse CD117 c-Kit antibody Cat. No. 570567/570655; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of CD117 c-Kit or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD117 c-Kit expression on Mouse bone marrow cells. BALB/c Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD45R/B220 Cat. No. 553090/561878 and with either BD Horizon RB705 Rat IgG2b, kappa Isotype Control Cat. No. 570263; Left Plot or BD Horizon RB705 Rat Anti-Mouse CD117 c-Kit antibody Cat. No. 570567/570655; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of CD117 c-Kit or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.