BD HorizonTM RB705 Rat Anti-Mouse CD335 (NKp46)

Description

Multicolor flow cytometric analysis of CD335 NKp46 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with PE Mouse Anti-Mouse NK-1.1 antibody Cat. No. 553165 and with either BD Horizon RB705 Rat IgG2a, kappa Isotype Control Cat. No. 570262; Left Plot or BD Horizon RB705 Rat Anti-Mouse CD335 NKp46 antibody Cat. No. 570565/570653; Right Plot at 1.0 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD335 NKp46 or Ig Isotype control staining versus NK1.1 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD335 NKp46 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with PE Mouse Anti-Mouse NK-1.1 antibody Cat. No. 553165 and with either BD Horizon RB705 Rat IgG2a, kappa Isotype Control Cat. No. 570262; Left Plot or BD Horizon RB705 Rat Anti-Mouse CD335 NKp46 antibody Cat. No. 570565/570653; Right Plot at 1.0 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD335 NKp46 or Ig Isotype control staining versus NK1.1 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD335 NKp46 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with PE Mouse Anti-Mouse NK-1.1 antibody Cat. No. 553165 and with either BD Horizon RB705 Rat IgG2a, kappa Isotype Control Cat. No. 570262; Left Plot or BD Horizon RB705 Rat Anti-Mouse CD335 NKp46 antibody Cat. No. 570565/570653; Right Plot at 1.0 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD335 NKp46 or Ig Isotype control staining versus NK1.1 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD335 NKp46 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with PE Mouse Anti-Mouse NK-1.1 antibody Cat. No. 553165 and with either BD Horizon RB705 Rat IgG2a, kappa Isotype Control Cat. No. 570262; Left Plot or BD Horizon RB705 Rat Anti-Mouse CD335 NKp46 antibody Cat. No. 570565/570653; Right Plot at 1.0 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD335 NKp46 or Ig Isotype control staining versus NK1.1 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD335 NKp46 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with PE Mouse Anti-Mouse NK-1.1 antibody Cat. No. 553165 and with either BD Horizon RB705 Rat IgG2a, kappa Isotype Control Cat. No. 570262; Left Plot or BD Horizon RB705 Rat Anti-Mouse CD335 NKp46 antibody Cat. No. 570565/570653; Right Plot at 1.0 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD335 NKp46 or Ig Isotype control staining versus NK1.1 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD335 NKp46 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with PE Mouse Anti-Mouse NK-1.1 antibody Cat. No. 553165 and with either BD Horizon RB705 Rat IgG2a, kappa Isotype Control Cat. No. 570262; Left Plot or BD Horizon RB705 Rat Anti-Mouse CD335 NKp46 antibody Cat. No. 570565/570653; Right Plot at 1.0 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD335 NKp46 or Ig Isotype control staining versus NK1.1 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.