BD HorizonTM RB705 Rat Anti-Mouse Ly-6C

Description

Two-color flow cytometric analysis of Ly-6C expression on Mouse splenic leukocytes. BALB/c Mouse splenic leukocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BUV395 Rat Anti-Mouse CD8a antibody Cat. No. 563786 and with either BD Horizon RB705 Rat IgM, kappa Isotype Control Cat. No. 570266; Left Plot or BD Horizon RB705 Rat Anti-Mouse Ly-6C antibody Cat. No. 570264/570265; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable leukocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Ly-6C expression on Mouse splenic leukocytes. BALB/c Mouse splenic leukocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BUV395 Rat Anti-Mouse CD8a antibody Cat. No. 563786 and with either BD Horizon RB705 Rat IgM, kappa Isotype Control Cat. No. 570266; Left Plot or BD Horizon RB705 Rat Anti-Mouse Ly-6C antibody Cat. No. 570264/570265; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable leukocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Ly-6C expression on Mouse splenic leukocytes. BALB/c Mouse splenic leukocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BUV395 Rat Anti-Mouse CD8a antibody Cat. No. 563786 and with either BD Horizon RB705 Rat IgM, kappa Isotype Control Cat. No. 570266; Left Plot or BD Horizon RB705 Rat Anti-Mouse Ly-6C antibody Cat. No. 570264/570265; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable leukocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of Ly-6C expression on Mouse splenic leukocytes. BALB/c Mouse splenic leukocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BUV395 Rat Anti-Mouse CD8a antibody Cat. No. 563786 and with either BD Horizon RB705 Rat IgM, kappa Isotype Control Cat. No. 570266; Left Plot or BD Horizon RB705 Rat Anti-Mouse Ly-6C antibody Cat. No. 570264/570265; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable leukocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of Ly-6C expression on Mouse splenic leukocytes. BALB/c Mouse splenic leukocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BUV395 Rat Anti-Mouse CD8a antibody Cat. No. 563786 and with either BD Horizon RB705 Rat IgM, kappa Isotype Control Cat. No. 570266; Left Plot or BD Horizon RB705 Rat Anti-Mouse Ly-6C antibody Cat. No. 570264/570265; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable leukocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Ly-6C expression on Mouse splenic leukocytes. BALB/c Mouse splenic leukocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BUV395 Rat Anti-Mouse CD8a antibody Cat. No. 563786 and with either BD Horizon RB705 Rat IgM, kappa Isotype Control Cat. No. 570266; Left Plot or BD Horizon RB705 Rat Anti-Mouse Ly-6C antibody Cat. No. 570264/570265; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable leukocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.