

570949 · 100 Tests
BDB Bioscience · Cat: 570949
Reach 3+ verified distributors
Manufacturer product page
bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow cytometric analysis of CD107a LAMP-1 expression in Jurkat cells. Cells from the Human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control Cat. No. 570519; dashed line histogram or BD Horizon RB744 Mouse Anti-Human CD107a LAMP-1 antibody Cat. No. 570949/571020; solid line histogram . The fluorescence histogram showing CD107a LAMP-1 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact Jurkat cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Flow cytometric analysis of CD107a LAMP-1 expression in Jurkat cells. Cells from the Human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control Cat. No. 570519; dashed line histogram or BD Horizon RB744 Mouse Anti-Human CD107a LAMP-1 antibody Cat. No. 570949/571020; solid line histogram . The fluorescence histogram showing CD107a LAMP-1 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact Jurkat cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Show More Flow cytometric analysis of CD107a LAMP-1 expression in Jurkat cells. Cells from the Human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control Cat. No. 570519; dashed line histogram or BD Horizon RB744 Mouse Anti-Human CD107a LAMP-1 antibody Cat. No. 570949/571020; solid line histogram . The fluorescence histogram showing CD107a LAMP-1 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact Jurkat cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software.