

570996 · 25 Tests
BDB Bioscience · Cat: 570996
Reach 3+ verified distributors
Manufacturer product page
bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Multiparameter flow cytometric analysis of CD196 CCR6 expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and stained with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control Cat. No. 570519; Left Plot or BD Horizon RB744 Mouse Anti-Human CD196 CCR6 antibody Cat. No. 570924/570996; Right Plot . The bivariate pseudocolor density plot showing CD196 CCR6 expression or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with the side and forward light-scatter characteristics of viable leukocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD196 CCR6 expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and stained with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control Cat. No. 570519; Left Plot or BD Horizon RB744 Mouse Anti-Human CD196 CCR6 antibody Cat. No. 570924/570996; Right Plot . The bivariate pseudocolor density plot showing CD196 CCR6 expression or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with the side and forward light-scatter characteristics of viable leukocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD196 CCR6 expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and stained with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control Cat. No. 570519; Left Plot or BD Horizon RB744 Mouse Anti-Human CD196 CCR6 antibody Cat. No. 570924/570996; Right Plot . The bivariate pseudocolor density plot showing CD196 CCR6 expression or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with the side and forward light-scatter characteristics of viable leukocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of CD196 CCR6 expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and stained with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control Cat. No. 570519; Left Plot or BD Horizon RB744 Mouse Anti-Human CD196 CCR6 antibody Cat. No. 570924/570996; Right Plot . The bivariate pseudocolor density plot showing CD196 CCR6 expression or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with the side and forward light-scatter characteristics of viable leukocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of CD196 CCR6 expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and stained with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control Cat. No. 570519; Left Plot or BD Horizon RB744 Mouse Anti-Human CD196 CCR6 antibody Cat. No. 570924/570996; Right Plot . The bivariate pseudocolor density plot showing CD196 CCR6 expression or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with the side and forward light-scatter characteristics of viable leukocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD196 CCR6 expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and stained with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control Cat. No. 570519; Left Plot or BD Horizon RB744 Mouse Anti-Human CD196 CCR6 antibody Cat. No. 570924/570996; Right Plot . The bivariate pseudocolor density plot showing CD196 CCR6 expression or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with the side and forward light-scatter characteristics of viable leukocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software.