BD HorizonTM RB744 Mouse Anti-Human CD207 (Langerin)

Description

Multicolor flow cytometric analysis of CD207 Langerin expression by monocyte-derived Langerhans cells. Human peripheral blood monocytes were cultured with Recombinant Human GM-CSF Cat. No. 550068; 100 ng/ml , IL-4 Cat. No. 554605; 20 ng/ml , and TGF-beta Corning; Cat. No. 354039; 40 ng/ml proteins for 3 days followed by an additional 3-day culture with GM-CSF and TGF-beta. The cells were washed and stained with BD Horizon BV421 Mouse Anti-Human CD1a antibody Cat. No. 563938 and BD Horizon Fixable Viability Stain 510 Cat. No. 564406 . After washing, the cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were subsequently washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control Cat. No. 570519; Left Plot or BD Horizon RB744 Mouse Anti-Human CD207 Langerin antibody Cat. No. 570587/570675; Right Plot . The bivariate pseudocolor density plots showing the correlated expression of CD207 Langerin or Ig Isotype control staining versus CD1a were derived from gated events with the forward and side light-scatter, and viability stain characteristics of intact cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Multicolor flow cytometric analysis of CD207 Langerin expression by monocyte-derived Langerhans cells. Human peripheral blood monocytes were cultured with Recombinant Human GM-CSF Cat. No. 550068; 100 ng/ml , IL-4 Cat. No. 554605; 20 ng/ml , and TGF-beta Corning; Cat. No. 354039; 40 ng/ml proteins for 3 days followed by an additional 3-day culture with GM-CSF and TGF-beta. The cells were washed and stained with BD Horizon BV421 Mouse Anti-Human CD1a antibody Cat. No. 563938 and BD Horizon Fixable Viability Stain 510 Cat. No. 564406 . After washing, the cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were subsequently washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control Cat. No. 570519; Left Plot or BD Horizon RB744 Mouse Anti-Human CD207 Langerin antibody Cat. No. 570587/570675; Right Plot . The bivariate pseudocolor density plots showing the correlated expression of CD207 Langerin or Ig Isotype control staining versus CD1a were derived from gated events with the forward and side light-scatter, and viability stain characteristics of intact cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Multicolor flow cytometric analysis of CD207 Langerin expression by monocyte-derived Langerhans cells. Human peripheral blood monocytes were cultured with Recombinant Human GM-CSF Cat. No. 550068; 100 ng/ml , IL-4 Cat. No. 554605; 20 ng/ml , and TGF-beta Corning; Cat. No. 354039; 40 ng/ml proteins for 3 days followed by an additional 3-day culture with GM-CSF and TGF-beta. The cells were washed and stained with BD Horizon BV421 Mouse Anti-Human CD1a antibody Cat. No. 563938 and BD Horizon Fixable Viability Stain 510 Cat. No. 564406 . After washing, the cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were subsequently washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control Cat. No. 570519; Left Plot or BD Horizon RB744 Mouse Anti-Human CD207 Langerin antibody Cat. No. 570587/570675; Right Plot . The bivariate pseudocolor density plots showing the correlated expression of CD207 Langerin or Ig Isotype control staining versus CD1a were derived from gated events with the forward and side light-scatter, and viability stain characteristics of intact cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Show More Multicolor flow cytometric analysis of CD207 Langerin expression by monocyte-derived Langerhans cells. Human peripheral blood monocytes were cultured with Recombinant Human GM-CSF Cat. No. 550068; 100 ng/ml , IL-4 Cat. No. 554605; 20 ng/ml , and TGF-beta Corning; Cat. No. 354039; 40 ng/ml proteins for 3 days followed by an additional 3-day culture with GM-CSF and TGF-beta. The cells were washed and stained with BD Horizon BV421 Mouse Anti-Human CD1a antibody Cat. No. 563938 and BD Horizon Fixable Viability Stain 510 Cat. No. 564406 . After washing, the cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were subsequently washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control Cat. No. 570519; Left Plot or BD Horizon RB744 Mouse Anti-Human CD207 Langerin antibody Cat. No. 570587/570675; Right Plot . The bivariate pseudocolor density plots showing the correlated expression of CD207 Langerin or Ig Isotype control staining versus CD1a were derived from gated events with the forward and side light-scatter, and viability stain characteristics of intact cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Show More Multicolor flow cytometric analysis of CD207 Langerin expression by monocyte-derived Langerhans cells. Human peripheral blood monocytes were cultured with Recombinant Human GM-CSF Cat. No. 550068; 100 ng/ml , IL-4 Cat. No. 554605; 20 ng/ml , and TGF-beta Corning; Cat. No. 354039; 40 ng/ml proteins for 3 days followed by an additional 3-day culture with GM-CSF and TGF-beta. The cells were washed and stained with BD Horizon BV421 Mouse Anti-Human CD1a antibody Cat. No. 563938 and BD Horizon Fixable Viability Stain 510 Cat. No. 564406 . After washing, the cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were subsequently washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control Cat. No. 570519; Left Plot or BD Horizon RB744 Mouse Anti-Human CD207 Langerin antibody Cat. No. 570587/570675; Right Plot . The bivariate pseudocolor density plots showing the correlated expression of CD207 Langerin or Ig Isotype control staining versus CD1a were derived from gated events with the forward and side light-scatter, and viability stain characteristics of intact cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Multicolor flow cytometric analysis of CD207 Langerin expression by monocyte-derived Langerhans cells. Human peripheral blood monocytes were cultured with Recombinant Human GM-CSF Cat. No. 550068; 100 ng/ml , IL-4 Cat. No. 554605; 20 ng/ml , and TGF-beta Corning; Cat. No. 354039; 40 ng/ml proteins for 3 days followed by an additional 3-day culture with GM-CSF and TGF-beta. The cells were washed and stained with BD Horizon BV421 Mouse Anti-Human CD1a antibody Cat. No. 563938 and BD Horizon Fixable Viability Stain 510 Cat. No. 564406 . After washing, the cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were subsequently washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control Cat. No. 570519; Left Plot or BD Horizon RB744 Mouse Anti-Human CD207 Langerin antibody Cat. No. 570587/570675; Right Plot . The bivariate pseudocolor density plots showing the correlated expression of CD207 Langerin or Ig Isotype control staining versus CD1a were derived from gated events with the forward and side light-scatter, and viability stain characteristics of intact cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software.