BD HorizonTM RB744 Mouse Anti-Human CD25 (IL-2 Receptor alpha)

Description

Flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated and PHA-stimulated Human peripheral blood lymphocytes Left and Middle Plots - Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424/562425 and with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control Cat. No. 570519; Left Plot or BD Horizon RB744 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 570620/570708; Middle Plot . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Right Plot - Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RB744 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated and PHA-stimulated Human peripheral blood lymphocytes Left and Middle Plots - Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424/562425 and with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control Cat. No. 570519; Left Plot or BD Horizon RB744 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 570620/570708; Middle Plot . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Right Plot - Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RB744 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated and PHA-stimulated Human peripheral blood lymphocytes Left and Middle Plots - Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424/562425 and with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control Cat. No. 570519; Left Plot or BD Horizon RB744 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 570620/570708; Middle Plot . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Right Plot - Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RB744 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Show More Flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated and PHA-stimulated Human peripheral blood lymphocytes Left and Middle Plots - Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424/562425 and with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control Cat. No. 570519; Left Plot or BD Horizon RB744 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 570620/570708; Middle Plot . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Right Plot - Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RB744 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Show More Flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated and PHA-stimulated Human peripheral blood lymphocytes Left and Middle Plots - Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424/562425 and with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control Cat. No. 570519; Left Plot or BD Horizon RB744 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 570620/570708; Middle Plot . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Right Plot - Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RB744 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated and PHA-stimulated Human peripheral blood lymphocytes Left and Middle Plots - Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424/562425 and with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control Cat. No. 570519; Left Plot or BD Horizon RB744 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 570620/570708; Middle Plot . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Right Plot - Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RB744 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software.