

570656 · 25 Tests
BDB Bioscience · Cat: 570656
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow cytometric analysis of CD275 ICOS Ligand expression on B7-H2-transfected CHO cells. Human B7-H2-transfected Chinese Hamster Ovary CHO cells were stained with either BD Horizon RB744 Mouse IgG2b, kappa Isotype Control Cat. No. 570523; dashed line histogram or BD Horizon RB744 Mouse Anti-Human CD275 ICOS Ligand antibody Cat. No. 570568/570656; solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD275 ICOS Ligand expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Flow cytometric analysis of CD275 ICOS Ligand expression on B7-H2-transfected CHO cells. Human B7-H2-transfected Chinese Hamster Ovary CHO cells were stained with either BD Horizon RB744 Mouse IgG2b, kappa Isotype Control Cat. No. 570523; dashed line histogram or BD Horizon RB744 Mouse Anti-Human CD275 ICOS Ligand antibody Cat. No. 570568/570656; solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD275 ICOS Ligand expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Flow cytometric analysis of CD275 ICOS Ligand expression on B7-H2-transfected CHO cells. Human B7-H2-transfected Chinese Hamster Ovary CHO cells were stained with either BD Horizon RB744 Mouse IgG2b, kappa Isotype Control Cat. No. 570523; dashed line histogram or BD Horizon RB744 Mouse Anti-Human CD275 ICOS Ligand antibody Cat. No. 570568/570656; solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD275 ICOS Ligand expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.