BD HorizonTM RB744 Mouse Anti-Human CD335 (NKp46)

Description

Multiparameter flow cytometric analysis of CD335 NKp46 expression on Human peripheral blood leukocytes. Human whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 . The leukocytes were then stained with Alexa Fluor 647 Mouse Anti-Human NCAM-1 CD56 antibody Cat. No. 563443; Top Plots and with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control Cat. No. 570519; Left Plots or BD Horizon RB744 Mouse Anti-Human CD335 NKp46 antibody Cat. No. 570586/570674; Right Plots . Bivariate pseudocolor density plots showing the correlated expression patterns of either CD335 NKp46 or Ig Isotype control staining versus NCAM-1 CD56 Top Plots or side light-scatter SSC-A signals Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes Top Plots or leukocyte populations Bottom Plots . Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD335 NKp46 expression on Human peripheral blood leukocytes. Human whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 . The leukocytes were then stained with Alexa Fluor 647 Mouse Anti-Human NCAM-1 CD56 antibody Cat. No. 563443; Top Plots and with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control Cat. No. 570519; Left Plots or BD Horizon RB744 Mouse Anti-Human CD335 NKp46 antibody Cat. No. 570586/570674; Right Plots . Bivariate pseudocolor density plots showing the correlated expression patterns of either CD335 NKp46 or Ig Isotype control staining versus NCAM-1 CD56 Top Plots or side light-scatter SSC-A signals Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes Top Plots or leukocyte populations Bottom Plots . Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of CD335 NKp46 expression on Human peripheral blood leukocytes. Human whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 . The leukocytes were then stained with Alexa Fluor 647 Mouse Anti-Human NCAM-1 CD56 antibody Cat. No. 563443; Top Plots and with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control Cat. No. 570519; Left Plots or BD Horizon RB744 Mouse Anti-Human CD335 NKp46 antibody Cat. No. 570586/570674; Right Plots . Bivariate pseudocolor density plots showing the correlated expression patterns of either CD335 NKp46 or Ig Isotype control staining versus NCAM-1 CD56 Top Plots or side light-scatter SSC-A signals Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes Top Plots or leukocyte populations Bottom Plots . Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software.