BD HorizonTM RB744 Mouse Anti-Human CD39 (ENTPD1)

Description

Multiparameter flow cytometric analysis of CD39 ENTPD1 expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and stained with either BD Horizon RB744 Mouse IgG2b, kappa Isotype Control Cat. No. 570523; Left Plot or BD Horizon RB744 Mouse Anti-Human CD39 ENTPD1 antibody Cat. No. 570637/570723; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD39 ENTPD1 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo software. Multicolor flow cytometric analysis of CD39 ENTPD1 expression on Human peripheral blood lymphocytes. Human peripheral blood was stained with BD Horizon BV421 Mouse Anti-Human CD4 Cat. No. 562424 , Alexa Fluor 700 Mouse Anti-Human CD25 Cat. No. 561398 , and PE Anti-Human CD127 Cat. No. 557938 antibodies, and with either BD Horizon RB744 Mouse IgG2b, kappa Isotype Control dashed line histogram; Right Plot or BD Horizon RB744 Mouse Anti-Human CD39 ENTPD1 antibody solid line histogram; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The fluorescence histogram showing CD39 ENTPD1 expression or Ig Isotype control staining Right Plot was derived from CD4+CD25+CD127low gated events ie, cells with a Regulatory T cell immunophenotype; Left Plot with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD39 ENTPD1 expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and stained with either BD Horizon RB744 Mouse IgG2b, kappa Isotype Control Cat. No. 570523; Left Plot or BD Horizon RB744 Mouse Anti-Human CD39 ENTPD1 antibody Cat. No. 570637/570723; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD39 ENTPD1 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo software. Show More Multicolor flow cytometric analysis of CD39 ENTPD1 expression on Human peripheral blood lymphocytes. Human peripheral blood was stained with BD Horizon BV421 Mouse Anti-Human CD4 Cat. No. 562424 , Alexa Fluor 700 Mouse Anti-Human CD25 Cat. No. 561398 , and PE Anti-Human CD127 Cat. No. 557938 antibodies, and with either BD Horizon RB744 Mouse IgG2b, kappa Isotype Control dashed line histogram; Right Plot or BD Horizon RB744 Mouse Anti-Human CD39 ENTPD1 antibody solid line histogram; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The fluorescence histogram showing CD39 ENTPD1 expression or Ig Isotype control staining Right Plot was derived from CD4+CD25+CD127low gated events ie, cells with a Regulatory T cell immunophenotype; Left Plot with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of CD39 ENTPD1 expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and stained with either BD Horizon RB744 Mouse IgG2b, kappa Isotype Control Cat. No. 570523; Left Plot or BD Horizon RB744 Mouse Anti-Human CD39 ENTPD1 antibody Cat. No. 570637/570723; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD39 ENTPD1 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo software. Multicolor flow cytometric analysis of CD39 ENTPD1 expression on Human peripheral blood lymphocytes. Human peripheral blood was stained with BD Horizon BV421 Mouse Anti-Human CD4 Cat. No. 562424 , Alexa Fluor 700 Mouse Anti-Human CD25 Cat. No. 561398 , and PE Anti-Human CD127 Cat. No. 557938 antibodies, and with either BD Horizon RB744 Mouse IgG2b, kappa Isotype Control dashed line histogram; Right Plot or BD Horizon RB744 Mouse Anti-Human CD39 ENTPD1 antibody solid line histogram; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The fluorescence histogram showing CD39 ENTPD1 expression or Ig Isotype control staining Right Plot was derived from CD4+CD25+CD127low gated events ie, cells with a Regulatory T cell immunophenotype; Left Plot with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.