BD HorizonTM RB744 Mouse Anti-Human CD45RO

Description

Multiparameter flow cytometric analysis of CD45RO expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and stained with BD Horizon BV421 Mouse Anti-Human CD45RA antibody Cat. No. 562885 and with either BD Horizon RB744 Mouse IgG2a, kappa Isotype Control Cat. No. 570518; Left Plots or BD Horizon RB744 Mouse Anti-Human CD45RO antibody Cat. No. 570638/570724; Right Plots . Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Top Plots: The bivariate pseudocolor density plots showing CD45RO expression or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the side and forward light-scatter characteristics of viable leukocytes. Bottom Plots: The bivariate pseudocolor density plots showing CD45RO expression or Ig isotype control staining versus CD45RA were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Multiparameter flow cytometric analysis of CD45RO expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and stained with BD Horizon BV421 Mouse Anti-Human CD45RA antibody Cat. No. 562885 and with either BD Horizon RB744 Mouse IgG2a, kappa Isotype Control Cat. No. 570518; Left Plots or BD Horizon RB744 Mouse Anti-Human CD45RO antibody Cat. No. 570638/570724; Right Plots . Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Top Plots: The bivariate pseudocolor density plots showing CD45RO expression or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the side and forward light-scatter characteristics of viable leukocytes. Bottom Plots: The bivariate pseudocolor density plots showing CD45RO expression or Ig isotype control staining versus CD45RA were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Show More Multiparameter flow cytometric analysis of CD45RO expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and stained with BD Horizon BV421 Mouse Anti-Human CD45RA antibody Cat. No. 562885 and with either BD Horizon RB744 Mouse IgG2a, kappa Isotype Control Cat. No. 570518; Left Plots or BD Horizon RB744 Mouse Anti-Human CD45RO antibody Cat. No. 570638/570724; Right Plots . Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Top Plots: The bivariate pseudocolor density plots showing CD45RO expression or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the side and forward light-scatter characteristics of viable leukocytes. Bottom Plots: The bivariate pseudocolor density plots showing CD45RO expression or Ig isotype control staining versus CD45RA were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.