BD HorizonTM RB744 Mouse Anti-Human Granzyme B

Description

Two-color flow cytometric analysis of Granzyme B expression in Human peripheral blood lymphocytes. Human peripheral blood cells were treated 15 min; room temperature with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Mouse Anti-Human CD8 antibody Cat. No. 561953 and with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control Cat. No. 570519; Left Plot or BD Horizon RB744 Mouse Anti-Human Granzyme B antibody Cat. No. 570498/570499; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme B or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Two-color flow cytometric analysis of Granzyme B expression in Human peripheral blood lymphocytes. Human peripheral blood cells were treated 15 min; room temperature with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Mouse Anti-Human CD8 antibody Cat. No. 561953 and with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control Cat. No. 570519; Left Plot or BD Horizon RB744 Mouse Anti-Human Granzyme B antibody Cat. No. 570498/570499; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme B or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Two-color flow cytometric analysis of Granzyme B expression in Human peripheral blood lymphocytes. Human peripheral blood cells were treated 15 min; room temperature with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Mouse Anti-Human CD8 antibody Cat. No. 561953 and with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control Cat. No. 570519; Left Plot or BD Horizon RB744 Mouse Anti-Human Granzyme B antibody Cat. No. 570498/570499; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme B or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Show More Two-color flow cytometric analysis of Granzyme B expression in Human peripheral blood lymphocytes. Human peripheral blood cells were treated 15 min; room temperature with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Mouse Anti-Human CD8 antibody Cat. No. 561953 and with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control Cat. No. 570519; Left Plot or BD Horizon RB744 Mouse Anti-Human Granzyme B antibody Cat. No. 570498/570499; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme B or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Show More Two-color flow cytometric analysis of Granzyme B expression in Human peripheral blood lymphocytes. Human peripheral blood cells were treated 15 min; room temperature with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Mouse Anti-Human CD8 antibody Cat. No. 561953 and with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control Cat. No. 570519; Left Plot or BD Horizon RB744 Mouse Anti-Human Granzyme B antibody Cat. No. 570498/570499; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme B or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Two-color flow cytometric analysis of Granzyme B expression in Human peripheral blood lymphocytes. Human peripheral blood cells were treated 15 min; room temperature with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Mouse Anti-Human CD8 antibody Cat. No. 561953 and with either BD Horizon RB744 Mouse IgG1, kappa Isotype Control Cat. No. 570519; Left Plot or BD Horizon RB744 Mouse Anti-Human Granzyme B antibody Cat. No. 570498/570499; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme B or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software.