BD HorizonTM RB744 Mouse Anti-Human Perforin

Description

Flow cytometric analysis of Perforin expression in Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed with and stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon RB744 Mouse IgG2b, kappa Isotype Control Cat. No. 570523; dashed line histogram or BD Horizon RB744 Mouse Anti-Human Perforin antibody Cat. No. 570592/570680; solid line histogram . The fluorescence histogram showing Perforin expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Flow cytometric analysis of Perforin expression in Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed with and stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon RB744 Mouse IgG2b, kappa Isotype Control Cat. No. 570523; dashed line histogram or BD Horizon RB744 Mouse Anti-Human Perforin antibody Cat. No. 570592/570680; solid line histogram . The fluorescence histogram showing Perforin expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Show More Flow cytometric analysis of Perforin expression in Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed with and stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon RB744 Mouse IgG2b, kappa Isotype Control Cat. No. 570523; dashed line histogram or BD Horizon RB744 Mouse Anti-Human Perforin antibody Cat. No. 570592/570680; solid line histogram . The fluorescence histogram showing Perforin expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software.