BD HorizonTM RB744 Rat Anti-CD11b (Integrin alphaM)

Description

Multiparameter flow cytometric analysis of CD11b Integrin alphaM expression on viable Mouse bone marrow cells. BALB/c Mouse bone-marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon RB744 Rat IgG2b, kappa Isotype Control Cat. No. 570521; Left Plot or BD Horizon RB744 Rat Anti-CD11b Integrin alphaM antibody Cat. No. 570512/570513; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD11b Integrin alphaM or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD11b Integrin alphaM expression on Human peripheral blood leukocyte populations. Human whole blood was stained with either BD Horizon RB744 Rat IgG2b, kappa Isotype Control Cat. No. 570521; Left Plot or BD Horizon RB744 Rat Anti-CD11b Integrin alphaM antibody Cat. No. 570512/570513; Right Plot at 0.25 mug/test. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD11b Integrin alphaM or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD11b Integrin alphaM expression on viable Mouse bone marrow cells. BALB/c Mouse bone-marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon RB744 Rat IgG2b, kappa Isotype Control Cat. No. 570521; Left Plot or BD Horizon RB744 Rat Anti-CD11b Integrin alphaM antibody Cat. No. 570512/570513; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD11b Integrin alphaM or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD11b Integrin alphaM expression on Human peripheral blood leukocyte populations. Human whole blood was stained with either BD Horizon RB744 Rat IgG2b, kappa Isotype Control Cat. No. 570521; Left Plot or BD Horizon RB744 Rat Anti-CD11b Integrin alphaM antibody Cat. No. 570512/570513; Right Plot at 0.25 mug/test. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD11b Integrin alphaM or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD11b Integrin alphaM expression on viable Mouse bone marrow cells. BALB/c Mouse bone-marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon RB744 Rat IgG2b, kappa Isotype Control Cat. No. 570521; Left Plot or BD Horizon RB744 Rat Anti-CD11b Integrin alphaM antibody Cat. No. 570512/570513; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD11b Integrin alphaM or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD11b Integrin alphaM expression on Human peripheral blood leukocyte populations. Human whole blood was stained with either BD Horizon RB744 Rat IgG2b, kappa Isotype Control Cat. No. 570521; Left Plot or BD Horizon RB744 Rat Anti-CD11b Integrin alphaM antibody Cat. No. 570512/570513; Right Plot at 0.25 mug/test. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD11b Integrin alphaM or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.