BD HorizonTM RB744 Rat Anti-Mouse CD117 (c-Kit)

Description

Multiparameter flow cytometric analysis of CD117 c-Kit expression on Mouse bone marrow cells. C57BL/6 Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 . The cells were then stained with PE Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553090 and with either BD Horizon RB744 Rat IgG2b, kappa Isotype Control Cat. No. 570523; Left Plot or BD Horizon RB744 Rat Anti-Mouse CD117 c-Kit antibody Cat. No. 570552/570556; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD117 c-Kit or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD117 c-Kit expression on Mouse bone marrow cells. C57BL/6 Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 . The cells were then stained with PE Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553090 and with either BD Horizon RB744 Rat IgG2b, kappa Isotype Control Cat. No. 570523; Left Plot or BD Horizon RB744 Rat Anti-Mouse CD117 c-Kit antibody Cat. No. 570552/570556; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD117 c-Kit or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD117 c-Kit expression on Mouse bone marrow cells. C57BL/6 Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 . The cells were then stained with PE Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553090 and with either BD Horizon RB744 Rat IgG2b, kappa Isotype Control Cat. No. 570523; Left Plot or BD Horizon RB744 Rat Anti-Mouse CD117 c-Kit antibody Cat. No. 570552/570556; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD117 c-Kit or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD117 c-Kit expression on Mouse bone marrow cells. C57BL/6 Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 . The cells were then stained with PE Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553090 and with either BD Horizon RB744 Rat IgG2b, kappa Isotype Control Cat. No. 570523; Left Plot or BD Horizon RB744 Rat Anti-Mouse CD117 c-Kit antibody Cat. No. 570552/570556; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD117 c-Kit or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD117 c-Kit expression on Mouse bone marrow cells. C57BL/6 Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 . The cells were then stained with PE Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553090 and with either BD Horizon RB744 Rat IgG2b, kappa Isotype Control Cat. No. 570523; Left Plot or BD Horizon RB744 Rat Anti-Mouse CD117 c-Kit antibody Cat. No. 570552/570556; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD117 c-Kit or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD117 c-Kit expression on Mouse bone marrow cells. C57BL/6 Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 . The cells were then stained with PE Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553090 and with either BD Horizon RB744 Rat IgG2b, kappa Isotype Control Cat. No. 570523; Left Plot or BD Horizon RB744 Rat Anti-Mouse CD117 c-Kit antibody Cat. No. 570552/570556; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD117 c-Kit or Ig Isotype control staining versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.