BD HorizonTM RB744 Rat Anti-Mouse CD3

Description

Multicolor flow cytometric analysis of CD3 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse spleen cells were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The leukocytes were then stained with PE Rat Anti-Mouse CD19 antibody Cat. No. 561736 and with either BD Horizon RB744 Rat IgG2b, kappa Isotype Control Cat. No. 570521; Left Plot or BD Horizon RB744 Rat Anti-Mouse CD3 antibody Cat. No. 570561/570649; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD3 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD3 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse spleen cells were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The leukocytes were then stained with PE Rat Anti-Mouse CD19 antibody Cat. No. 561736 and with either BD Horizon RB744 Rat IgG2b, kappa Isotype Control Cat. No. 570521; Left Plot or BD Horizon RB744 Rat Anti-Mouse CD3 antibody Cat. No. 570561/570649; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD3 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD3 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse spleen cells were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The leukocytes were then stained with PE Rat Anti-Mouse CD19 antibody Cat. No. 561736 and with either BD Horizon RB744 Rat IgG2b, kappa Isotype Control Cat. No. 570521; Left Plot or BD Horizon RB744 Rat Anti-Mouse CD3 antibody Cat. No. 570561/570649; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD3 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD3 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse spleen cells were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The leukocytes were then stained with PE Rat Anti-Mouse CD19 antibody Cat. No. 561736 and with either BD Horizon RB744 Rat IgG2b, kappa Isotype Control Cat. No. 570521; Left Plot or BD Horizon RB744 Rat Anti-Mouse CD3 antibody Cat. No. 570561/570649; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD3 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD3 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse spleen cells were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The leukocytes were then stained with PE Rat Anti-Mouse CD19 antibody Cat. No. 561736 and with either BD Horizon RB744 Rat IgG2b, kappa Isotype Control Cat. No. 570521; Left Plot or BD Horizon RB744 Rat Anti-Mouse CD3 antibody Cat. No. 570561/570649; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD3 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD3 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse spleen cells were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The leukocytes were then stained with PE Rat Anti-Mouse CD19 antibody Cat. No. 561736 and with either BD Horizon RB744 Rat IgG2b, kappa Isotype Control Cat. No. 570521; Left Plot or BD Horizon RB744 Rat Anti-Mouse CD3 antibody Cat. No. 570561/570649; Right Plot at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD3 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.