BD HorizonTM RB744 Rat Anti-Mouse CD45

Description

Flow cytometric analysis of CD45 expression on viable Mouse splenic lymphocytes. C57BL/6 Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon RB744 Rat IgG2b, kappa Isotype Control Cat. No. 570521; dashed line histogram or BD Horizon RB744 Rat Anti-Mouse CD45 antibody Cat. No. 570510/570511; solid line histogram at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD45 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD45 expression on viable Mouse splenic lymphocytes. C57BL/6 Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon RB744 Rat IgG2b, kappa Isotype Control Cat. No. 570521; dashed line histogram or BD Horizon RB744 Rat Anti-Mouse CD45 antibody Cat. No. 570510/570511; solid line histogram at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD45 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD45 expression on viable Mouse splenic lymphocytes. C57BL/6 Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon RB744 Rat IgG2b, kappa Isotype Control Cat. No. 570521; dashed line histogram or BD Horizon RB744 Rat Anti-Mouse CD45 antibody Cat. No. 570510/570511; solid line histogram at 0.125 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD45 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.