BD HorizonTM RB744 Rat Anti-Mouse CD62L (L-Selectin)

Description

Multicolor flow cytometric analysis of CD62L L-Selectin expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and with either BD Horizon RB744 Rat IgG2a, kappa Isotype Control Left Plot or BD Horizon RB744 Rat Anti-Mouse CD62L L-Selectin antibody Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD62L L-Selectin or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD62L L-Selectin expression on viable Mouse bone marrow cells. C57BL/6 Mouse bone marrow cells were left untreated Left Plot or were cultured 1 hour with Phorbol 12-Myristate 13-Acetate PMA; Right Plot . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and then stained with either BD Horizon RB744 Rat IgG2a, kappa Isotype Control Cat. No. 570520; dashed line histograms or BD Horizon RB744 Rat Anti-Mouse CD62L L-Selectin antibody Cat. No. 570965/571036; solid line histograms at 0.25 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing CD62L L-Selectin expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD62L L-Selectin expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and with either BD Horizon RB744 Rat IgG2a, kappa Isotype Control Left Plot or BD Horizon RB744 Rat Anti-Mouse CD62L L-Selectin antibody Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD62L L-Selectin or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD62L L-Selectin expression on viable Mouse bone marrow cells. C57BL/6 Mouse bone marrow cells were left untreated Left Plot or were cultured 1 hour with Phorbol 12-Myristate 13-Acetate PMA; Right Plot . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and then stained with either BD Horizon RB744 Rat IgG2a, kappa Isotype Control Cat. No. 570520; dashed line histograms or BD Horizon RB744 Rat Anti-Mouse CD62L L-Selectin antibody Cat. No. 570965/571036; solid line histograms at 0.25 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing CD62L L-Selectin expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD62L L-Selectin expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and with either BD Horizon RB744 Rat IgG2a, kappa Isotype Control Left Plot or BD Horizon RB744 Rat Anti-Mouse CD62L L-Selectin antibody Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD62L L-Selectin or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD62L L-Selectin expression on viable Mouse bone marrow cells. C57BL/6 Mouse bone marrow cells were left untreated Left Plot or were cultured 1 hour with Phorbol 12-Myristate 13-Acetate PMA; Right Plot . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and then stained with either BD Horizon RB744 Rat IgG2a, kappa Isotype Control Cat. No. 570520; dashed line histograms or BD Horizon RB744 Rat Anti-Mouse CD62L L-Selectin antibody Cat. No. 570965/571036; solid line histograms at 0.25 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing CD62L L-Selectin expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.