BD HorizonTM RB744 Rat Anti-Mouse F4/80

Description

Multicolor flow cytometric analysis of F4/80 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The leukocytes were then stained with APC Rat Anti-CD11b antibody Cat. No. 553312 and with either BD Horizon RB744 Rat IgG2a, kappa Isotype Control Cat. No. 570520; Left Plot or BD Horizon RB744 Rat Anti-Mouse F4/80 antibody Cat. No. 570508/570509; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative monocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of F4/80 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The leukocytes were then stained with APC Rat Anti-CD11b antibody Cat. No. 553312 and with either BD Horizon RB744 Rat IgG2a, kappa Isotype Control Cat. No. 570520; Left Plot or BD Horizon RB744 Rat Anti-Mouse F4/80 antibody Cat. No. 570508/570509; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative monocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of F4/80 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The leukocytes were then stained with APC Rat Anti-CD11b antibody Cat. No. 553312 and with either BD Horizon RB744 Rat IgG2a, kappa Isotype Control Cat. No. 570520; Left Plot or BD Horizon RB744 Rat Anti-Mouse F4/80 antibody Cat. No. 570508/570509; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative monocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of F4/80 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The leukocytes were then stained with APC Rat Anti-CD11b antibody Cat. No. 553312 and with either BD Horizon RB744 Rat IgG2a, kappa Isotype Control Cat. No. 570520; Left Plot or BD Horizon RB744 Rat Anti-Mouse F4/80 antibody Cat. No. 570508/570509; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative monocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of F4/80 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The leukocytes were then stained with APC Rat Anti-CD11b antibody Cat. No. 553312 and with either BD Horizon RB744 Rat IgG2a, kappa Isotype Control Cat. No. 570520; Left Plot or BD Horizon RB744 Rat Anti-Mouse F4/80 antibody Cat. No. 570508/570509; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative monocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of F4/80 expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The leukocytes were then stained with APC Rat Anti-CD11b antibody Cat. No. 553312 and with either BD Horizon RB744 Rat IgG2a, kappa Isotype Control Cat. No. 570520; Left Plot or BD Horizon RB744 Rat Anti-Mouse F4/80 antibody Cat. No. 570508/570509; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative monocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.