BD HorizonTM RB780 Mouse Anti-Bcl-6

Description

Multicolor flow cytometric analysis of Bcl-6 expression in Mouse B lymphocytes. BALB/c Mouse mesenteric lymph node cells were stained with APC Rat Anti-Mouse CD45R/B220 Cat. No. 553092 , BD OptiBuild BUV395 Rat Anti-Mouse CD4 Cat. No. 740208 , BD Horizon BB515 Hamster Anti-Mouse CD95 Cat. No. 565605 , and BD OptiBuild BV421 Rat Anti-Mouse IgD Cat. No. 744291 antibodies. Cells were washed, resuspended in RPMI 1640 medium, and fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 . Cells were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 followed by intracellular staining with BD Horizon RB780 Mouse Anti-Bcl-6 antibody Cat. No. 569142/569143 . The bivariate pseudocolor density plot showing the correlated expression of IgD versus Fas/CD95 on B cells was derived from CD4-B220+ gated events with the forward and side light-scatter characteristics of intact lymphocytes Left Plot . Germinal center GC B cells were identified as Fas/CD95-positive B cells that expressed low levels of IgD IgDloCD95/Fas+ whereas non-GC B cells primarily expressed intermediate to high levels of IgD and little or no CD95/Fas. Histograms Right Plot derived from gated cell subsets show intracellular Bcl-6 staining levels for Mouse GC B cells solid line and non-GC B cells dashed line . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of Bcl-6 expression in Human Ramos cells. Human Ramos cells Burkitts lymphoma, ATCC CRL-1596 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 followed by washing and intracellular staining with BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; dashed line histogram or BD Horizon RB780 Mouse Anti-Bcl-6 antibody Cat. No. 569142/569143; solid line histogram . The histogram showing Bcl-6 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multicolor flow cytometric analysis of Bcl-6 expression in Mouse B lymphocytes. BALB/c Mouse mesenteric lymph node cells were stained with APC Rat Anti-Mouse CD45R/B220 Cat. No. 553092 , BD OptiBuild BUV395 Rat Anti-Mouse CD4 Cat. No. 740208 , BD Horizon BB515 Hamster Anti-Mouse CD95 Cat. No. 565605 , and BD OptiBuild BV421 Rat Anti-Mouse IgD Cat. No. 744291 antibodies. Cells were washed, resuspended in RPMI 1640 medium, and fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 . Cells were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 followed by intracellular staining with BD Horizon RB780 Mouse Anti-Bcl-6 antibody Cat. No. 569142/569143 . The bivariate pseudocolor density plot showing the correlated expression of IgD versus Fas/CD95 on B cells was derived from CD4-B220+ gated events with the forward and side light-scatter characteristics of intact lymphocytes Left Plot . Germinal center GC B cells were identified as Fas/CD95-positive B cells that expressed low levels of IgD IgDloCD95/Fas+ whereas non-GC B cells primarily expressed intermediate to high levels of IgD and little or no CD95/Fas. Histograms Right Plot derived from gated cell subsets show intracellular Bcl-6 staining levels for Mouse GC B cells solid line and non-GC B cells dashed line . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of Bcl-6 expression in Human Ramos cells. Human Ramos cells Burkitts lymphoma, ATCC CRL-1596 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 followed by washing and intracellular staining with BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; dashed line histogram or BD Horizon RB780 Mouse Anti-Bcl-6 antibody Cat. No. 569142/569143; solid line histogram . The histogram showing Bcl-6 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multicolor flow cytometric analysis of Bcl-6 expression in Mouse B lymphocytes. BALB/c Mouse mesenteric lymph node cells were stained with APC Rat Anti-Mouse CD45R/B220 Cat. No. 553092 , BD OptiBuild BUV395 Rat Anti-Mouse CD4 Cat. No. 740208 , BD Horizon BB515 Hamster Anti-Mouse CD95 Cat. No. 565605 , and BD OptiBuild BV421 Rat Anti-Mouse IgD Cat. No. 744291 antibodies. Cells were washed, resuspended in RPMI 1640 medium, and fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 . Cells were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 followed by intracellular staining with BD Horizon RB780 Mouse Anti-Bcl-6 antibody Cat. No. 569142/569143 . The bivariate pseudocolor density plot showing the correlated expression of IgD versus Fas/CD95 on B cells was derived from CD4-B220+ gated events with the forward and side light-scatter characteristics of intact lymphocytes Left Plot . Germinal center GC B cells were identified as Fas/CD95-positive B cells that expressed low levels of IgD IgDloCD95/Fas+ whereas non-GC B cells primarily expressed intermediate to high levels of IgD and little or no CD95/Fas. Histograms Right Plot derived from gated cell subsets show intracellular Bcl-6 staining levels for Mouse GC B cells solid line and non-GC B cells dashed line . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of Bcl-6 expression in Human Ramos cells. Human Ramos cells Burkitts lymphoma, ATCC CRL-1596 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 followed by washing and intracellular staining with BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; dashed line histogram or BD Horizon RB780 Mouse Anti-Bcl-6 antibody Cat. No. 569142/569143; solid line histogram . The histogram showing Bcl-6 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.