BD HorizonTM RB780 Mouse Anti-Bcl-6

Description

Multicolor flow cytometric analysis of Bcl-6 expression in Mouse B lymphocytes. BALB/c Mouse mesenteric lymph node cells were stained with APC Rat Anti-Mouse CD45R/B220 Cat. No. 553092 , BD OptiBuild BUV395 Rat Anti-Mouse CD4 Cat. No. 740208 , BD Horizon BB515 Hamster Anti-Mouse CD95 Cat. No. 565605 , and BD OptiBuild BV421 Rat Anti-Mouse IgD Cat. No. 744291 antibodies. Cells were washed, resuspended in RPMI 1640 medium, and fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 . Cells were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 followed by intracellular staining with BD Horizon RB780 Mouse Anti-Bcl-6 antibody Cat. No. 569142/569143 . The bivariate pseudocolor density plot showing the correlated expression of IgD versus Fas/CD95 on B cells was derived from CD4-B220+ gated events with the forward and side light-scatter characteristics of intact lymphocytes Left Plot . Germinal center GC B cells were identified as Fas/CD95-positive B cells that expressed low levels of IgD IgDloCD95/Fas+ whereas non-GC B cells primarily expressed intermediate to high levels of IgD and little or no CD95/Fas. Histograms Right Plot derived from gated cell subsets show intracellular Bcl-6 staining levels for Mouse GC B cells solid line and non-GC B cells dashed line . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multicolor flow cytometric analysis of Bcl-6 expression in Mouse B lymphocytes. BALB/c Mouse mesenteric lymph node cells were stained with APC Rat Anti-Mouse CD45R/B220 Cat. No. 553092 , BD OptiBuild BUV395 Rat Anti-Mouse CD4 Cat. No. 740208 , BD Horizon BB515 Hamster Anti-Mouse CD95 Cat. No. 565605 , and BD OptiBuild BV421 Rat Anti-Mouse IgD Cat. No. 744291 antibodies. Cells were washed, resuspended in RPMI 1640 medium, and fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 . Cells were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 followed by intracellular staining with BD Horizon RB780 Mouse Anti-Bcl-6 antibody Cat. No. 569142/569143 . The bivariate pseudocolor density plot showing the correlated expression of IgD versus Fas/CD95 on B cells was derived from CD4-B220+ gated events with the forward and side light-scatter characteristics of intact lymphocytes Left Plot . Germinal center GC B cells were identified as Fas/CD95-positive B cells that expressed low levels of IgD IgDloCD95/Fas+ whereas non-GC B cells primarily expressed intermediate to high levels of IgD and little or no CD95/Fas. Histograms Right Plot derived from gated cell subsets show intracellular Bcl-6 staining levels for Mouse GC B cells solid line and non-GC B cells dashed line . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multicolor flow cytometric analysis of Bcl-6 expression in Mouse B lymphocytes. BALB/c Mouse mesenteric lymph node cells were stained with APC Rat Anti-Mouse CD45R/B220 Cat. No. 553092 , BD OptiBuild BUV395 Rat Anti-Mouse CD4 Cat. No. 740208 , BD Horizon BB515 Hamster Anti-Mouse CD95 Cat. No. 565605 , and BD OptiBuild BV421 Rat Anti-Mouse IgD Cat. No. 744291 antibodies. Cells were washed, resuspended in RPMI 1640 medium, and fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 . Cells were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 followed by intracellular staining with BD Horizon RB780 Mouse Anti-Bcl-6 antibody Cat. No. 569142/569143 . The bivariate pseudocolor density plot showing the correlated expression of IgD versus Fas/CD95 on B cells was derived from CD4-B220+ gated events with the forward and side light-scatter characteristics of intact lymphocytes Left Plot . Germinal center GC B cells were identified as Fas/CD95-positive B cells that expressed low levels of IgD IgDloCD95/Fas+ whereas non-GC B cells primarily expressed intermediate to high levels of IgD and little or no CD95/Fas. Histograms Right Plot derived from gated cell subsets show intracellular Bcl-6 staining levels for Mouse GC B cells solid line and non-GC B cells dashed line . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.