BD HorizonTM RB780 Mouse Anti-Human CCR7 (CD197)

Description

Multicolor flow cytometric analysis of CCR7 CD197 on Human peripheral blood leucocytes and lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD4 Cat. No. 561841 , BD Horizon BUV395 Mouse Anti-Human CD8 Cat. No. 563795/563796 , BD Horizon BV421 Mouse Anti-Human CD45RA Cat. No. 562885 antibodies, and with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532 or BD Horizon RB780 Mouse Anti-Human CCR7 CD197 antibody Cat. No. 568748/568749 . The erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . Flow cytometry and data analysis were performed using a BD FACS LSRFortessa Flow Cytometer and FlowJo software. Plots 1 and 2: The bivariate pseudocolor density plots showing the correlated expression of CCR7 CD197 or Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Plot 3: The two-parameter pseudocolor density plot showing the correlated expression of CCR7 CD197 versus CD45RA was derived from CD4-positive T cell gated events with the forward and side light-scatter characteristics of viable lymphocytes. Please note that we have observed considerable donor-to-donor variation in the proportion of CCR7 CD197 -negative CD4-positive lymphocytes. Plot 4: The two-parameter pseudocolor density plot showing CCR7 CD197 staining versus CD45RA was derived from CD8-positive T cell gated events with the forward and side light-scatter characteristics of viable lymphocytes. Multicolor flow cytometric analysis of CCR7 CD197 on Human peripheral blood leucocytes and lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD4 Cat. No. 561841 , BD Horizon BUV395 Mouse Anti-Human CD8 Cat. No. 563795/563796 , BD Horizon BV421 Mouse Anti-Human CD45RA Cat. No. 562885 antibodies, and with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532 or BD Horizon RB780 Mouse Anti-Human CCR7 CD197 antibody Cat. No. 568748/568749 . The erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . Flow cytometry and data analysis were performed using a BD FACS LSRFortessa Flow Cytometer and FlowJo software. Plots 1 and 2: The bivariate pseudocolor density plots showing the correlated expression of CCR7 CD197 or Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Plot 3: The two-parameter pseudocolor density plot showing the correlated expression of CCR7 CD197 versus CD45RA was derived from CD4-positive T cell gated events with the forward and side light-scatter characteristics of viable lymphocytes. Please note that we have observed considerable donor-to-donor variation in the proportion of CCR7 CD197 -negative CD4-positive lymphocytes. Plot 4: The two-parameter pseudocolor density plot showing CCR7 CD197 staining versus CD45RA was derived from CD8-positive T cell gated events with the forward and side light-scatter characteristics of viable lymphocytes. Show More Multicolor flow cytometric analysis of CCR7 CD197 on Human peripheral blood leucocytes and lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD4 Cat. No. 561841 , BD Horizon BUV395 Mouse Anti-Human CD8 Cat. No. 563795/563796 , BD Horizon BV421 Mouse Anti-Human CD45RA Cat. No. 562885 antibodies, and with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532 or BD Horizon RB780 Mouse Anti-Human CCR7 CD197 antibody Cat. No. 568748/568749 . The erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . Flow cytometry and data analysis were performed using a BD FACS LSRFortessa Flow Cytometer and FlowJo software. Plots 1 and 2: The bivariate pseudocolor density plots showing the correlated expression of CCR7 CD197 or Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Plot 3: The two-parameter pseudocolor density plot showing the correlated expression of CCR7 CD197 versus CD45RA was derived from CD4-positive T cell gated events with the forward and side light-scatter characteristics of viable lymphocytes. Please note that we have observed considerable donor-to-donor variation in the proportion of CCR7 CD197 -negative CD4-positive lymphocytes. Plot 4: The two-parameter pseudocolor density plot showing CCR7 CD197 staining versus CD45RA was derived from CD8-positive T cell gated events with the forward and side light-scatter characteristics of viable lymphocytes.