BD HorizonTM RB780 Mouse Anti-Human CD103 (Integrin alphaE)

Description

Multiparameter flow cytometric analysis of CD103 Integrin alphaE expression on Human peripheral blood leucocytes. Human whole blood was stained with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; Left Plot or with BD Horizon RB780 Mouse Anti-Human CD103 Integrin alphaE antibody Cat. No. 569331/569332; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . A bivariate pseudocolor density plot showing the correlated expression of CD103 Integrin alphaE or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD103 Integrin alphaE expression on activated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated with Phytohemagglutinin PHA; 3 days . The cells were then stained with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RB780 Mouse Anti-Human CD103 Integrin alphaE antibody solid line histogram . The fluorescence histogram showing the expressed levels of CD103 Integrin alphaE or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Multiparameter flow cytometric analysis of CD103 Integrin alphaE expression on Human peripheral blood leucocytes. Human whole blood was stained with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; Left Plot or with BD Horizon RB780 Mouse Anti-Human CD103 Integrin alphaE antibody Cat. No. 569331/569332; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . A bivariate pseudocolor density plot showing the correlated expression of CD103 Integrin alphaE or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of CD103 Integrin alphaE expression on activated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated with Phytohemagglutinin PHA; 3 days . The cells were then stained with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RB780 Mouse Anti-Human CD103 Integrin alphaE antibody solid line histogram . The fluorescence histogram showing the expressed levels of CD103 Integrin alphaE or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Show More Multiparameter flow cytometric analysis of CD103 Integrin alphaE expression on Human peripheral blood leucocytes. Human whole blood was stained with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; Left Plot or with BD Horizon RB780 Mouse Anti-Human CD103 Integrin alphaE antibody Cat. No. 569331/569332; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . A bivariate pseudocolor density plot showing the correlated expression of CD103 Integrin alphaE or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD103 Integrin alphaE expression on activated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated with Phytohemagglutinin PHA; 3 days . The cells were then stained with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RB780 Mouse Anti-Human CD103 Integrin alphaE antibody solid line histogram . The fluorescence histogram showing the expressed levels of CD103 Integrin alphaE or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes.