BD HorizonTM RB780 Mouse Anti-Human CD152

Description

Multicolor flow cytometric analysis of CD152 expression on activated Human peripheral blood lymphocytes. Concanavalin A Con A -activated 3 days Human peripheral blood mononuclear cells PBMC were preincubated with Human BD Fc Block Cat. No. 564219/564220 . These cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/561810 and with either BD Horizon RB780 Mouse IgG2a, kappa Isotype Control Cat. No. 568740; Left Plot or BD Horizon RB780 Mouse Anti-Human CD152 antibody Cat. No. 569335/569336; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD152 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multicolor flow cytometric analysis of CD152 expression on activated Human peripheral blood lymphocytes. Concanavalin A Con A -activated 3 days Human peripheral blood mononuclear cells PBMC were preincubated with Human BD Fc Block Cat. No. 564219/564220 . These cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/561810 and with either BD Horizon RB780 Mouse IgG2a, kappa Isotype Control Cat. No. 568740; Left Plot or BD Horizon RB780 Mouse Anti-Human CD152 antibody Cat. No. 569335/569336; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD152 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multicolor flow cytometric analysis of CD152 expression on activated Human peripheral blood lymphocytes. Concanavalin A Con A -activated 3 days Human peripheral blood mononuclear cells PBMC were preincubated with Human BD Fc Block Cat. No. 564219/564220 . These cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/561810 and with either BD Horizon RB780 Mouse IgG2a, kappa Isotype Control Cat. No. 568740; Left Plot or BD Horizon RB780 Mouse Anti-Human CD152 antibody Cat. No. 569335/569336; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD152 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multicolor flow cytometric analysis of CD152 expression on activated Human peripheral blood lymphocytes. Concanavalin A Con A -activated 3 days Human peripheral blood mononuclear cells PBMC were preincubated with Human BD Fc Block Cat. No. 564219/564220 . These cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/561810 and with either BD Horizon RB780 Mouse IgG2a, kappa Isotype Control Cat. No. 568740; Left Plot or BD Horizon RB780 Mouse Anti-Human CD152 antibody Cat. No. 569335/569336; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD152 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multicolor flow cytometric analysis of CD152 expression on activated Human peripheral blood lymphocytes. Concanavalin A Con A -activated 3 days Human peripheral blood mononuclear cells PBMC were preincubated with Human BD Fc Block Cat. No. 564219/564220 . These cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/561810 and with either BD Horizon RB780 Mouse IgG2a, kappa Isotype Control Cat. No. 568740; Left Plot or BD Horizon RB780 Mouse Anti-Human CD152 antibody Cat. No. 569335/569336; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD152 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multicolor flow cytometric analysis of CD152 expression on activated Human peripheral blood lymphocytes. Concanavalin A Con A -activated 3 days Human peripheral blood mononuclear cells PBMC were preincubated with Human BD Fc Block Cat. No. 564219/564220 . These cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/561810 and with either BD Horizon RB780 Mouse IgG2a, kappa Isotype Control Cat. No. 568740; Left Plot or BD Horizon RB780 Mouse Anti-Human CD152 antibody Cat. No. 569335/569336; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD152 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.