BD HorizonTM RB780 Mouse Anti-Human CD25 (IL-2 Receptor alpha)

Description

Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated Human leukocytes Left Plots and stimulated Right Plot Human lymphocytes. Left Plots - Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424 and with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; Left Plot or BD Horizon RB780 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 570327/570403; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Right Plot - Peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RB780 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated Human leukocytes Left Plots and stimulated Right Plot Human lymphocytes. Left Plots - Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424 and with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; Left Plot or BD Horizon RB780 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 570327/570403; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Right Plot - Peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RB780 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated Human leukocytes Left Plots and stimulated Right Plot Human lymphocytes. Left Plots - Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424 and with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; Left Plot or BD Horizon RB780 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 570327/570403; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Right Plot - Peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RB780 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated Human leukocytes Left Plots and stimulated Right Plot Human lymphocytes. Left Plots - Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424 and with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; Left Plot or BD Horizon RB780 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 570327/570403; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Right Plot - Peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RB780 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated Human leukocytes Left Plots and stimulated Right Plot Human lymphocytes. Left Plots - Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424 and with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; Left Plot or BD Horizon RB780 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 570327/570403; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Right Plot - Peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RB780 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated Human leukocytes Left Plots and stimulated Right Plot Human lymphocytes. Left Plots - Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD4 antibody Cat. No. 562424 and with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; Left Plot or BD Horizon RB780 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 570327/570403; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Right Plot - Peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RB780 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.