BD HorizonTM RB780 Mouse Anti-Human CD39 (ENTPD1)

Description

Flow cytometric analysis of CD39 ENTPD1 expression on Human peripheral blood leukocyte populations. Upper Plots: Human whole blood was stained with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat No. 568532; Left Plot or BD Horizon RB780 Mouse Anti-Human CD39 ENTPD1 antibody Cat. No. 570042/570123; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD39 ENTPD1 or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Lower Plots: Human peripheral blood mononuclear cells PBMC were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with Alexa Fluor 488 Mouse Anti-Human CD4 Cat. No. 557695 , Alexa Fluor 700 Mouse Anti-Human CD25 Cat. No. 561398 , PE Mouse Anti-Human CD127 Cat No. 557938 antibodies, and with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RB780 Mouse Anti-Human CD39 ENTPD1 antibody solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the coexpressed levels of CD25 versus CD127 by viable DAPI-negative light scatter-gated CD4+ T cells Left Plot was further gated to reveal CD39 ENTPD1 expression or Ig Isotype control staining Right Plot on CD4+CD25+CD127low T cells i.e., cells with a Regulatory T cell immunophenotype as shown. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Flow cytometric analysis of CD39 ENTPD1 expression on Human peripheral blood leukocyte populations. Upper Plots: Human whole blood was stained with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat No. 568532; Left Plot or BD Horizon RB780 Mouse Anti-Human CD39 ENTPD1 antibody Cat. No. 570042/570123; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD39 ENTPD1 or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Lower Plots: Human peripheral blood mononuclear cells PBMC were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with Alexa Fluor 488 Mouse Anti-Human CD4 Cat. No. 557695 , Alexa Fluor 700 Mouse Anti-Human CD25 Cat. No. 561398 , PE Mouse Anti-Human CD127 Cat No. 557938 antibodies, and with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RB780 Mouse Anti-Human CD39 ENTPD1 antibody solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the coexpressed levels of CD25 versus CD127 by viable DAPI-negative light scatter-gated CD4+ T cells Left Plot was further gated to reveal CD39 ENTPD1 expression or Ig Isotype control staining Right Plot on CD4+CD25+CD127low T cells i.e., cells with a Regulatory T cell immunophenotype as shown. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Show More Flow cytometric analysis of CD39 ENTPD1 expression on Human peripheral blood leukocyte populations. Upper Plots: Human whole blood was stained with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat No. 568532; Left Plot or BD Horizon RB780 Mouse Anti-Human CD39 ENTPD1 antibody Cat. No. 570042/570123; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD39 ENTPD1 or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Lower Plots: Human peripheral blood mononuclear cells PBMC were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with Alexa Fluor 488 Mouse Anti-Human CD4 Cat. No. 557695 , Alexa Fluor 700 Mouse Anti-Human CD25 Cat. No. 561398 , PE Mouse Anti-Human CD127 Cat No. 557938 antibodies, and with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RB780 Mouse Anti-Human CD39 ENTPD1 antibody solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the coexpressed levels of CD25 versus CD127 by viable DAPI-negative light scatter-gated CD4+ T cells Left Plot was further gated to reveal CD39 ENTPD1 expression or Ig Isotype control staining Right Plot on CD4+CD25+CD127low T cells i.e., cells with a Regulatory T cell immunophenotype as shown. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software.