BD HorizonTM RB780 Mouse Anti-Human CD40 Ligand (CD154)

Description

Flow cytometric analysis of CD40 Ligand CD154 expression on stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 4 hours with 20 ng/ml Phorbol 12-Myristate 13-Acetate PMA; Sigma-Aldrich Cat. No. P-8139 and 250 ng/ml Calcium Ionophore A23187 Sigma-Aldrich Cat. No. C-9275 . The cells were then preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; dashed line histogram or BD Horizon RB780 Mouse Anti-Human CD40 Ligand CD154 antibody Cat. No. 569325/569326; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD40 Ligand CD154 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD40 Ligand CD154 expression on stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 4 hours with 20 ng/ml Phorbol 12-Myristate 13-Acetate PMA; Sigma-Aldrich Cat. No. P-8139 and 250 ng/ml Calcium Ionophore A23187 Sigma-Aldrich Cat. No. C-9275 . The cells were then preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; dashed line histogram or BD Horizon RB780 Mouse Anti-Human CD40 Ligand CD154 antibody Cat. No. 569325/569326; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD40 Ligand CD154 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of CD40 Ligand CD154 expression on stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 4 hours with 20 ng/ml Phorbol 12-Myristate 13-Acetate PMA; Sigma-Aldrich Cat. No. P-8139 and 250 ng/ml Calcium Ionophore A23187 Sigma-Aldrich Cat. No. C-9275 . The cells were then preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; dashed line histogram or BD Horizon RB780 Mouse Anti-Human CD40 Ligand CD154 antibody Cat. No. 569325/569326; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD40 Ligand CD154 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.