BD HorizonTM RB780 Mouse Anti-Human Chromogranin A

Description

Flow cytometric analysis of Chromogranin A expression in Human neuroblastoma cells. Cells from the Human SH-SY5Y Neuroblastoma, ATCC CRL-2266 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed, permeabilized with and stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; dashed line histogram or BD Horizon RB780 Mouse Anti-Human Chromogranin A antibody Cat. No. 570813/570889; solid line histogram . The fluorescence histogram showing Chromogranin A expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact SH-SY5Y cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Flow cytometric analysis of Chromogranin A expression in Human neuroblastoma cells. Cells from the Human SH-SY5Y Neuroblastoma, ATCC CRL-2266 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed, permeabilized with and stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; dashed line histogram or BD Horizon RB780 Mouse Anti-Human Chromogranin A antibody Cat. No. 570813/570889; solid line histogram . The fluorescence histogram showing Chromogranin A expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact SH-SY5Y cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Show More Flow cytometric analysis of Chromogranin A expression in Human neuroblastoma cells. Cells from the Human SH-SY5Y Neuroblastoma, ATCC CRL-2266 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed, permeabilized with and stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; dashed line histogram or BD Horizon RB780 Mouse Anti-Human Chromogranin A antibody Cat. No. 570813/570889; solid line histogram . The fluorescence histogram showing Chromogranin A expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact SH-SY5Y cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software.